JAK1 (Human) antibody

Overview


	Antigen:	JAK1
	Antibody Type:	Polyclonal
	Application:	Western Blotting (WB)
	Reactivity:	Human, Mouse (Murine), Rat
	Host:	Rabbit
	Quantity:	0.1 ml
	Price:	360,00 € (Plus shipping costs and VAT)
	Order number:	ABIN113754
	Availability:	3 to 10 days until shipment

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Additional Information: JAK1 (Human) antibody

Antigen	JAK1
Immunogen	The antibody was produced using a synthetic peptide derived from an internal region of human JAK1. The sequence is conserved in mouse and rat.
Reactivity	Human, Mouse (Murine), Rat
Antibody Type	Polyclonal
Format	Aff - Purified
Host	Rabbit
Specificity	Janus Kinase 1 (JAK1) is a 130 kDa tyrosine kinase involved in cytoplasmic signal transduction. Ligand binding to a variety of cell surface receptors (e.g., cytokine, growth factor, GPCRs) leads to an association of those receptors with JAK proteins, which are then activated via phosphorylation of tyrosine residues in the kinase activation loop. Activated JAK proteins phosphorylate and activate STAT (signal transducers and activators of transcription) proteins, which then dimerize and translocate to the nucleus. Once in the nucleus, STAT proteins bind to DNA and modify the transcription of various genes, which can lead to various responses such as cell proliferation, cell survival and differentiation. This antibody is useful to determine total levels of JAK1 protein. Reacts with Human, mouse and rat JAK1.

Application Details: JAK1 (Human) antibody

Application	Western Blotting (WB)
Application Notes	The antibody has been used for Western blotting. For Western blotting applications, we recommend using the antibody at a 1:1000 dilution. Positive control used: 3T3-L1 adipocytes +/- Leukemia Inhibitory Factor (LIF). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Buffer	This antibody is supplied as liquid, purified IgG in Dulbecco’s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier and 0.05% sodium azide as preservative. Purification: P
Storage	Store at -20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment or short-term storage (up to one week), 2-8oC is sufficient.
Research Area	Signaling, Cytokines, Receptors, Kinases/Phosphatases
Restrictions	For Research Use only

Publications: JAK1 (Human) antibody

Publications

Schubert, Kuepper-Nybelen, Ihle et al.:
"[Utilization patterns of dementia patients in the light of statutory health insurance data]"
In: Zeitschrift für ärztliche Fortbildung und Qualitätssicherung : in Zusammenarbeit mit der Kaiserin-Friedrich-Stiftung für das ärztliche Fortbildungswesen , Vol. 101, Issue 1, pp. 7-13, 2007/01 (PubMed)

Xiang, Zhang, Yang et al.:
"[The efficacy and safety of long term home noninvasive positive pressure ventilation in patients with stable severe chronic obstructive pulmonary disease]"
In: Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases , Vol. 30, Issue 10, pp. 746-50, 2008/01 (PubMed)

Images: JAK1 (Human) antibody

Western Blot.,.Extracts prepared from 3T3-L1 cells stimulated with LIF were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with the JAK1 pan antibody for two hours at room temperature in a 1% BSA-TBST buffer. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP.,.conjugate and bands were detected using the Pierce SuperSignal™ method.

Image for JAK1 (Human) antibody (1)

External Information: JAK1 (Human) antibody

Google Scholar	Find more references for antigen JAK1 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen JAK1 (Bioinformatic Harvester (EMBL))