JAK1 antibody

Overview


	Antigen:	JAK1
	Antibody Type:	Polyclonal
	Application:	Western Blotting (WB)
	Reactivity:	Human
	Host:	Rabbit
	Quantity:	50 µl (5 WB) (Product variants)
	Price:	87,00 € (Plus shipping costs and VAT)
	Order number:	ABIN160746
	Availability:	Ships within 7 to 10 days

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Additional Information: JAK1 antibody

Antigen	JAK1
Immunogen	Recombinant (partial) , N-terminal
Reactivity	Human
Antibody Type	Polyclonal
Format	Purified
Isotype	Rabbit IgG »Matching secondary antibodies
Description	JAK1 (Janus kinase 1) a 130 kD non-receptor protein tyrosine kinase family and a member of the JAK subfamily is a widely expressed, membrane associated kinase involved in signal transduction pathways from interferon alpha, beta, and gamma receptors, all class II receptors, cytokine receptors that utilize the common gamma chain subunit for signaling, and cytokine receptors that utilize the gp130 subunit for signaling. JAK1 phosphorylates Stat proteins and SHP2. This kinase is constitutively expressed, but expression can be increased with interferon-alpha and interferon-gamma stimulation through transcription. JAK1 activity is increased by cytokine receptor-ligand binding and can be decreased by SOCS proteins, SHP2 protein phosphatase, and PPAR agonists. JAK1 is modified by phosphorylation and has been shown to associate with IFN-alpha receptor beta chain, IFN-gamma receptor alpha chain, IL-10 receptor alpha chain, IL-2 receptor beta chain, and IL-4 receptor alpha chain. The Poly6044 antibody recognizes the N-terminal region of human JAK1 and has been shown to be useful for Western blotting. Distribution: Widely expressed, membrane associated Function: Involved in signal transduction pathways from interferon alpha, beta, and gamma receptors, all class II receptors, cytokine receptors that utilize the common gamma chain subunit for signaling, and cytokine receptors that utilize the gp130 subunit for signaling, phosphorylates Stat proteins, SHP2 Modifications: Phosphorylation Synonyms: Janus kinase 1, JAK-1 Regulation: Constitutively expressed, can increase expression with interferon-alpha and -gamma stimulation through transcriptional activation. Activity increased by cytokine receptor-ligand binding, decreased by SOCS proteins, SHP2 protein phosphatase, and PPAR agonists Structure: Non-receptor protein tyrosine kinase family, JAK subfamily, 130 kD
Clone	Poly6044
Host	Rabbit
Specificity	Associates with IFN-alpha receptor beta chain, IFN-gamma receptor alpha chain, IL-10 receptor alpha chain, IL-2 receptor beta chain, IL-4 receptor alpha chain.

Application Details: JAK1 antibody

Application	Western Blotting (WB)
Application Notes	Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Purification	The antibody was purified by antigen-affinity chromatography.
Buffer	This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Storage	Upon receipt, store frozen at -20° C.
Research Area	Signaling, Cytokines, Receptors, Kinases/Phosphatases
Restrictions	For Research Use only

Publications: JAK1 antibody

Publications

Meraz, White, Sheehan et al.:
"Targeted disruption of the Stat1 gene in mice reveals unexpected physiologic specificity in the JAK-STAT signaling pathway."
In: Cell , Vol. 84, Issue 3, pp. 431-42, 1996/01 (PubMed)

Mueller, Briscoe, Laxton et al.:
"The protein tyrosine kinase JAK1 complements defects in interferon-alpha/beta and -gamma signal transduction."
In: Nature , Vol. 366, Issue 6451, pp. 129-35, 1993/01 (PubMed)

Rodig, Meraz, White et al.:
"Disruption of the Jak1 gene demonstrates obligatory and nonredundant roles of the Jaks in cytokine-induced biologic responses."
In: Cell , Vol. 93, Issue 3, pp. 373-83, 1998/01 (PubMed)

Wilks, Harpur, Kurban et al.:
"Two novel protein-tyrosine kinases, each with a second phosphotransferase-related catalytic domain, define a new class of protein kinase."
In: Molecular and cellular biology , Vol. 11, Issue 4, pp. 2057-65, 1991/01 (PubMed)

Images: JAK1 antibody

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-JAK-1 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

Image for JAK1 antibody (1)

Product variants

JAK1 antibody (Format: Purified, Quantity: 200 µl (20 WB))

External Information: JAK1 antibody

Google Scholar	Find more references for clone Poly6044 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen JAK1 (Bioinformatic Harvester (EMBL))