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Application
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Enzyme Immunoassay (EIA)
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Application Notes
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ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 1-2 ug/ml of this antibody will detect at least 0.03 ng/well of recombinant human MDC when used with PeproTech’s biotinylated antigen affinity purified anti-human MDC as the detection antibody at a concentration of at least 1 ug/ml. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
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Concentration
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1 mg/ml
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Buffer
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A sterile filtered antibody solution (1mg/ml) was lyophilized from PBS. This antibody was purified by Protein A affinity chromatography. Reconstituted in sterile water to a concentration of 1.0mg/ml.
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Storage
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The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at –20°C. When reconstituted in sterile water the antibody is stable for at least six weeks at 2-4°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Research Area
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Chemokines
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Restrictions
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For Research Use only
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