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Application
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Immunohistochemistry ((Frozen Sections) (IHC) (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Application Notes
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This antibody may be diluted to a titer of 1:20-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determinedby the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
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Purity
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This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
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Buffer
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Phosphate-buffered saline, pH7.2, containing 0.05% sodium azide
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Storage
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Store the antibody at 4-8 C. Do not use it beyond the expiration date stated on the label.
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Restrictions
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For research use only
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