Macrophage (F4/80) (Mouse) antibody

Overview

Antigen	Macrophage (F4/80)
Reactivity	Mouse (Murine)
Antibody Type	Monoclonal
Format	Purified
Isotype	IgG2b »Matching secondary antibodies
Description	Anti-mouse F4/80 monoclonal antibody reacts with the mouse macrophage F4/80 antigen, which is a 160kD plasma membrane component on mouse mononuclear phagocytes. The F4/80 antigen is found on most macrophages, and on macrophage precursors from M-CFC onward. Expression of this antigen is increased upon maturation. F4/80 is found in low levels on activated macrophages and eosinophils. Dendritic leukocytes may be negative or express F4/80 in low levels. Applications include flow cytometry. This clone is also reported to work in immunohistochemistry, both frozen and paraffin sections (#5).
Host	Rat
Specificity	Mouse Macrophage (F4/80)

Application	Flow Cytometry (FACS)
Concentration	0.2 mg/ml
Buffer	Purified Ig buffered in PBS and 0.1% NaN
Storage	Store at 4°C. For long term storage, aliquot and freeze unused portion at -20°C in volumes appropriate for single usage. Avoid freeze/thaw cycles. Check label for expiry date.
Restrictions	For Research Use only

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