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Product details for Macrophage Inflammatory Protein 1 alpha (Mouse) antibody
Macrophage Inflammatory Protein 1 alpha (Mouse) antibody
Overview
| Antigen: | Macrophage Inflammatory Protein 1 alpha |
| Antibody Type: | Polyclonal |
| Application: | ELISA, Neutralizing (Neut), Western Blotting (WB) |
| Reactivity: | Mouse (Murine) |
| Host: | Goat |
| Quantity: | |
| Price: | 247,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN147906 |
| Availability: | 7 to 10 days until shipment |
Additional Information: Macrophage Inflammatory Protein 1 alpha (Mouse) antibody
| Antigen | Macrophage Inflammatory Protein 1 alpha |
| Immunogen | Recombinant mouse macrophage inflammatory protein-1alpha, expressed in E. coli. |
| Reactivity | Mouse (Murine) |
| Antibody Type | Polyclonal |
| Format | Liquid |
| Isotype | IgG »Matching secondary antibodies |
| Description | MIP1 alpha belongs to the chemokine beta family. In vitro MIP1 alpha can synergize with the hematopoietic growth factors granulocyte macrophage CSF (GM-CSF) to enhance colony formation. |
| Host | Goat |
| Specificity | Anti-Mouse MIP1 alpha neutralizes the bioactivity of recombinant, mouse MIP1 alpha, but not that of recombinant, mouse MIP1 beta or recombinant, human MIP1 alpha. In indirect ELISA and immunoblotting, this antibody displays less than 5% cross reactivity w |
Application Details: Macrophage Inflammatory Protein 1 alpha (Mouse) antibody
| Application | ELISA, Neutralizing (Neut), Western Blotting (WB) |
| Application Notes | ELISA: Use at a concentration of 0.25 ug/ml to detect 1ng/well of recombinant, mouse MIP1 alpha. Neut: Use at a concentration of 3 ug/ml. WB: Use at a concentration of 1 ug/ml to detect 1ng/lane of recombinant mouse MIP1 alpha under non-reducing conditions. Predicted molecular weight: 11 kDa. Mouse MIP1 alpha was tested for its ability to neutralize the bioactivity of recombinant, mouse MIP1 alpha in a cell proliferation assay using hematopoietic stem cells. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of recombinant, mouse MIP1 alpha, which is present at five times its own EC50 (the concentration of recombinant, mouse MIP1 alpha producing a one-half maximal bioactivity without antibody). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
| Buffer | Preservative: NoneConstituents: PBS. pH 7.4 |
| Restrictions | For research use only |
External Information: Macrophage Inflammatory Protein 1 alpha (Mouse) antibody
| Google Scholar | Find more references for antigen Macrophage Inflammatory Protein 1 alpha on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Macrophage Inflammatory Protein 1 alpha (Bioinformatic Harvester (EMBL)) |
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