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Product details for Macrophages (Human) antibody
Macrophages (Human) antibody
Overview
| Antigen: | Macrophages |
| Antibody Type: | Monoclonal |
| Application: | Immunohistochemistry (Frozen Sections) (IHC (fro)) |
| Reactivity: | Human |
| Host: | Mouse |
| Quantity: | |
| Price: | 300,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN111911 |
| Availability: | 3 to 10 days until shipment |
Additional Information: Macrophages (Human) antibody
| Antigen | Macrophages |
| Immunogen | 24h cultivated human peritoneal macrophages |
| Reactivity | Human |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | IgG1 »Matching secondary antibodies |
| Clone | PM-2K |
| Host | Mouse |
| Specificity | PM-2K, together with X-4 and X-14, forms a particular group of macrophage specific antibodies which were classified at the V Leukocyte Typing Workshop held in Boston in 1993. PM-2K recognises tissue macrophages and macrophages in proliferative disorders of macrophages. PM-2K is one of the markers that, being negative on dendritic cells, can differentiate between macrophages and dendritic cells. This antibody reacts with human macrophages. positive on macrophages of cat, dog, pig, bovine and monkey negative in rabbit, rat, guinea pig, gold hamster, goat, horse. Under non-reducing conditions PM-2K recognizes an antigen of 150kD (Westernblot) which has been shown to be on cytoplasmic membranes. |
Application Details: Macrophages (Human) antibody
| Application | Immunohistochemistry (Frozen Sections) (IHC (fro)) |
| Application Notes | Immunohistochemistry on frozen sections: 0.5u g/ml (1:400). Fixation: Acetone (10 minutes at 4°C), 2 % periodate-lysine-paraformaldehyde 4 hours. Antigen Distribution: Isolated cells: Positive on >90% of alveolar macrophages, and on 10% of adherent peritoneal cells after 1 day culture Negative on freshly isolated blood monocytes, on freshly isolated peritoneal cells, and on bone marrow cells including monocytes, myelomonocytic precursors and megakaryocytes. Tissue sections: PM-2K stains most tissue macrophages in lymphoreticular organs such as thymus, spleen, lymph node and tonsil. It is positive on Kupffer cells of the liver, alveolar macrophages and macrophages in the interstitial tissues of the kidney, pancreas and many other organs. In Gaucher's disease multicentric reticulohistiocytosis and malignant histiocytosis proliferating macrophages are positive. In MFH infiltrating macrophages are stained but not tumor cells. Some bone stromal cells but not osteoclast-like multinucleated giant cells are stained in GCT. Microglial cells, osteoclasts and dendritic cells such as Langerhans cells, interdigitating cells and follicular dendritic cells are negative. Comparison of Staining Patterns of X-4, X-14 and PM-2K Tissue/Cell Type X-4 X-14 PM-2K Lymph nodes tingible body macrophages + - +Macrophages brain (microglial cells) + - +Blood Monocytes 24 hour culture + - - + = positive, + = weakly positive - = negative 1 Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Concentration | Lot specific |
| Buffer | This antibody (cell culture supernatant) was lyophilised from PBS buffer pH 7.2 with 0.01% Thimerosal and 0.01 % PMSF as preservatives and 10mg/ml BSA as stabilizer. Reconstitute in 0.5 ml distilled water (=stock solution). |
| Storage | Unreconstituted: 4°C for 1 year. After reconstitution: aliquot and freeze at -20°C or lower. Do not freeze working dilutions. Avoid repeated freezing and thawing. Stability of stock solution: 1 year at -70°C. Shelf life: one year from despatch. |
| Research Area | Cell/Tissue Markers, Inflammation |
| Restrictions | For Research Use only |
External Information: Macrophages (Human) antibody
| Google Scholar | Find more references for clone PM-2K on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Macrophages (Bioinformatic Harvester (EMBL)) |
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