Product details for  Myc Tag antibody
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Myc Tag antibody

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Antigen: Myc Tag
Antibody Type: Monoclonal
Application: Western Blotting (WB), Immunocytochemistry (ICC)
Conjugate: HRP
Host: Mouse
Quantity: 100 µL (Product variants)
Price: 216,00 €   (Plus shipping costs and VAT)
Order number: ABIN131874
Availability: 7 to 10 days until shipment
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Additional Information:  Myc Tag antibody

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Antigen Myc Tag
Immunogen This antibody was purified from hybridoma (clone PL14) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with GST-6myc-Tag fusion protein.
Antibody Type Monoclonal
Isotype IgG1 »Matching secondary antibodies
Description Epitope tagging is a widely accepted technique that involves fusing an epitope-containing peptide to a certain protein as a marker for gene expression. The gene expression can be easily monitored by Western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes the “tag epitope. Some amino acid sequences that are typically used for epitope tagging include YPYDVPDYA (HA-Tag), EQKLISEEDL (Myc-Tag) and YTDIEMNRLGK (VAV-G- Tag), which correspond to regions of the Influenza hemagglutinin protein, human c-myc gene product, and Vesicular stomatitis virus glycoprotein, respectively.
Clone PL14
Host Mouse
Specificity This antibody recognizes the Myc-Tag peptide sequence (EQKLISEEDL) by Western blotting and immunocytochemistry.

Application Details:  Myc Tag antibody

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Application Western Blotting (WB), Immunocytochemistry (ICC)
Application Notes Western blotting : 1:500 for chemiluminescence detection system. Immunohistochemistry: Not tested. Immunocytochemistry : 1:100
Conjugate HRP
Buffer In PBS containing 1% BSA, 10% glycerol and 0.01% p-hydroxyphenylacetic acid with preservative (0.15% Pro Clin150).
Research Area Tags/Labels
Restrictions For Research Use Only

Publications:  Myc Tag antibody

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Publications Fukumoto, Watanabe-Fukunaga, Fujisawa et al.:
"The fused protein kinase regulates Hedgehog-stimulated transcriptional activation in Drosophila Schneider 2 cells."
In: The Journal of biological chemistry , Vol. 276, Issue 42, pp. 38441-8, 2001/01 (PubMed)

Mukai, Hachiya, Shoji-Hoshino et al.:
"NADE, a p75NTR-associated cell death executor, is involved in signal transduction mediated by the common neurotrophin receptor p75NTR."
In: The Journal of biological chemistry , Vol. 275, Issue 23, pp. 17566-70, 2000/01 (PubMed)

Ohya, Kawasaki, Hiraga et al.:
"The DNA polymerase domain of pol(epsilon) is required for rapid, efficient, and highly accurate chromosomal DNA replication, telomere length maintenance, and normal cell senescence in Saccharomyces cerevisiae."
In: The Journal of biological chemistry , Vol. 277, Issue 31, pp. 28099-108, 2002/01 (PubMed)

Sasaki, Masuda, Iwai et al.:
"A RING finger protein Praja1 regulates Dlx5-dependent transcription through its ubiquitin ligase activity for the Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1."
In: The Journal of biological chemistry , Vol. 277, Issue 25, pp. 22541-6, 2002/01 (PubMed)

Hirota, Daitoku, Matsuzaki et al.:
"Hepatocyte nuclear factor-4 is a novel downstream target of insulin via FKHR as a signal-regulated transcriptional inhibitor."
In: The Journal of biological chemistry , Vol. 278, Issue 15, pp. 13056-60, 2003/01 (PubMed)

Satoh-Horikawa, Nakanishi, Takahashi et al.:
"Nectin-3, a new member of immunoglobulin-like cell adhesion molecules that shows homophilic and heterophilic cell-cell adhesion activities."
In: The Journal of biological chemistry , Vol. 275, Issue 14, pp. 10291-9, 2000/01 (PubMed)

Satoh-Horikawa, Nakanishi, Takahashi et al.:
In: (PubMed)

Tojo, Matsuzaki, Minami et al.:
"The aryl hydrocarbon receptor nuclear transporter is modulated by the SUMO-1 conjugation system."
In: The Journal of biological chemistry , Vol. 277, Issue 48, pp. 46576-85, 2002/01 (PubMed)

Images:  Myc Tag antibody

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Immunohistochemical detection of myc-tagged mouse TRAF1 expressed in 293T cells using ABIN131874

Image for Myc Tag antibody (1)

Western blot detection of N-terminal myc-tagged proteins, mouse Bad (1) and JNK1 (2) expressed in 293T cells using ABIN131874.

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External Information:  Myc Tag antibody

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Google Scholar Find more references for clone PL14 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen Myc Tag (Bioinformatic Harvester (EMBL))