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Product details for N-Acyl Mannosamine-1-Dehydrogenase antibody
N-Acyl Mannosamine-1-Dehydrogenase antibody
Overview
| Antigen: | N-Acyl Mannosamine-1-Dehydrogenase |
| Antibody Type: | Polyclonal |
| Application: | Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB) |
| Host: | Goat |
| Quantity: | |
| Price: | 350,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN116912 |
| Availability: | Ships in 3 to 10 days |
Additional Information: N-Acyl Mannosamine-1-Dehydrogenase antibody
| Antigen | N-Acyl Mannosamine-1-Dehydrogenase |
| Immunogen | N-Acyl Mannosamine-1-Dehydrogenase [E.coli Recombinant] |
| Antibody Type | Polyclonal |
| Format | Ig Fraction |
| Host | Goat |
| Specificity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified N-Acylmannosamine-1-Dehydrogenase [Pseudomonas]. Cross reactivity against N-Acylmannosamine-1-Dehydrogenase from other sources is unknown. |
Application Details: N-Acyl Mannosamine-1-Dehydrogenase antibody
| Application | Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB) |
| Application Notes | Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. This product has been assayed against 1.0 µg of N-Acylmannosamine-1-Dehydrogenase [Pseudomonas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&,L] (Rabbit) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. Other applications not tested. |
| Concentration | 10.0 mg/ml (by UV absorbance at 280 nm) |
| Buffer | This antibody was lyophilised from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative. |
| Storage | Store vial at 4° C prior to restoration. Restore with 5.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of restoration. |
| Research Area | Enzymes |
| Restrictions | Research only |
Product variants
| N-Acyl Mannosamine-1-Dehydrogenase antibody (Format: Serum, Quantity: 2 ml) |
| N-Acyl Mannosamine-1-Dehydrogenase antibody (Quantity: 10 mg, Conjugate: Biotin) |
External Information: N-Acyl Mannosamine-1-Dehydrogenase antibody
| Google Scholar | Find more references for antigen N-Acyl Mannosamine-1-Dehydrogenase on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen N-Acyl Mannosamine-1-Dehydrogenase (Bioinformatic Harvester (EMBL)) |
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