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Produktdetails für  N-Acylmannosamine-1-Dehydrogenase antibody

N-Acylmannosamine-1-Dehydrogenase antibody

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Antigen: N-Acylmannosamine-1-Dehydrogenase [Microbial]
Antikörper-Typ: Polyklonal
Applikation: Enzyme Immunoassay (EIA), Western Blotting (WB)
Konjugat: HRP
Wirt: Goat
Menge: 20 mg (10.0 mg/ml (by UV absorbance a...)
Preis: 360,00 €   (Zzgl. Versandkosten und MWSt)
Bestellnummer: ABIN117977
Verfügbarkeit: Ships in 3 to 10 days
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Produktbeschreibung:  N-Acylmannosamine-1-Dehydrogenase antibody

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Antigen N-Acylmannosamine-1-Dehydrogenase [Microbial]
Immunogen N-Acylmannosamine-1-Dehydrogenase [Pseudomonas]
Antikörper-Typ Polyklonal
Wirt Goat
Spezifität This product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, and purified and partially purified N-Acylmannosamine-1-Dehydrogenase derived from Pseudomonas.

Anwendungen:  N-Acylmannosamine-1-Dehydrogenase antibody

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Applikation Enzyme Immunoassay (EIA), Western Blotting (WB)
Applikationshinweise Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. This product has been assayed against 1.0 ug of N-Acylmannosamine-1-Dehydrogenase IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 is suggested for this product. Optimal titers for other applications should be determined by the researcher.
Konjugat HRP
Konzentration 10.0 mg/ml (by UV absorbance at 280 nm)
Buffer This antibody was conjugated to Horseradish Peroxidase (HRP) and lyophilised from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Gentamicin Sulfate as preservative and 10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease free
Lagerung Store vial at 4° C prior to restoration. Restore with 2.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Forschungsgebiet Enzymes
Beschränkungen Research only

Publikationen:  N-Acylmannosamine-1-Dehydrogenase antibody

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Publikationen Farr, Nakane:
"Immunohistochemistry with enzyme labeled antibodies: a brief review."
In: Journal of immunological methods , Vol. 47, Issue 2, pp. 129-44, 1982/01 (PubMed)

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