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Product details for Na+K ATPase beta (Dog (Canine)) antibody
Na+K ATPase beta (Dog (Canine)) antibody
Overview
| Antigen: | ATPase (Na/K) beta |
| Antibody Type: | Monoclonal |
| Application: | Immunohistochemistry (Frozen Sections) (IHC) (fro), Immunoprecipitation (IP), Western Blotting (WB), Immunoblotting (IB) |
| Reactivity: | Dog (Canine), Human, Pig (Porcine), Sheep (Ovine) |
| Host: | Mouse |
| Quantity: | |
| Price: | 108,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN197867 |
| Availability: | Ships within 1 day |
Additional Information: Na+K ATPase beta (Dog (Canine)) antibody
| Antigen | ATPase (Na/K) beta |
| Immunogen | Purified lamb kidney beta sodium/potassium ATPase. Epitope: aa 195-199 |
| Reactivity | Dog (Canine), Human, Pig (Porcine), Sheep (Ovine) |
| Antibody Type | Monoclonal |
| Format | Ascites |
| Isotype | IgG2a »Matching secondary antibodies |
| Description | The sodium/potassium ATPase is an integral membrane enzyme found in all cells ofhigher organisms and is responsible for the ATP-dependent transport of sodium andpotassium across the cell membrane. This membrane-bound enzyme is related to anumber of other ATPases including sarcoplasmic and endoplasmic reticulum calciumATPase (SERCA) and plasma membrane calcium ATPase (PMCA). Thesodium/potassium ATPase consists of a large, multipass, transmembrane catalyticsubunit, termed the alpha subunit, and an associated smaller glycoprotein, termed thebeta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1,alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded bytwo multigene families. The different isoforms of the sodium/potassium ATPase exhibit a tissue-specific anddevelopmental pattern of expression. The alpha 1 and beta mRNAs are present in allcell types examined whereas the alpha 2 and alpha 3 mRNAs exhibit a more restrictedpattern of cell-specific expression. |
| Clone | M17-P5-F11 |
| Host | Mouse |
| Specificity | Detects sodium/potassium ATPase beta 1. This antibody does not recognize the beta 1or beta 2 subunit in rat. Cross-reacts with Human, Dog, Pig and Sheep. Not yet tested in other species. |
Application Details: Na+K ATPase beta (Dog (Canine)) antibody
| Application | Immunohistochemistry (Frozen Sections) (IHC) (fro), Immunoprecipitation (IP), Western Blotting (WB), Immunoblotting (IB) |
| Application Notes | Suggested working dilutions *immunohistochemistry-Fr, immunoprecipitation, Western Blot This antibody can be used in Immunohistochemistry, Immunoprecipitation, and WesternBlot. By Western blot, this antibody detects an ~42 kDa protein in liver samples and~55 kDa in kidney samples representing beta sodium/potassium ATPase in sheep liverextract. Immunohistochemical staining of the sodium/potassium ATPase yields a patternconsistent with plasma membrane localization. Recommended Starting Dilutions*: IHC: 1/100 WB: 1/250Not tested in other applications.*Optimal dilutions should be determined by the end user. * Other applications have not been tested. |
| Buffer | PBS with 0.05% sodium azide 0.2 ml ascites. |
| Storage | Aliquot and store at -20 degrees C. Avoid repeated freeze / thaw cycles. |
| Restrictions | For Research Use only |
External Information: Na+K ATPase beta (Dog (Canine)) antibody
| Google Scholar | Find more references for clone M17-P5-F11 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen ATPase (Na/K) beta (Bioinformatic Harvester (EMBL)) |
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