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Product details for Nestin (Rat) antibody
Nestin (Rat) antibody
Overview
| Antigen: | Nestin |
| Antibody Type: | Monoclonal |
| Application: | Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB) |
| Reactivity: | Rat |
| Host: | Mouse |
| Quantity: | |
| Price: | 390,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN112422 |
| Availability: | 3 to 10 days until shipment |
Additional Information: Nestin (Rat) antibody
| Antigen | Nestin |
| Immunogen | Nestin purified from embryonic rat spinal cord. |
| Reactivity | Rat |
| Antibody Type | Monoclonal |
| Format | Ig Fraction |
| Isotype | IgG »Matching secondary antibodies |
| Clone | 4D4 |
| Host | Mouse |
| Specificity | Reacts with rat and mouse nestin. Does not react with human. |
Application Details: Nestin (Rat) antibody
| Application | Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB) |
| Application Notes | Immunohistochemistry: frozen sections. Antigen preservation: we recommend perfusing tissues with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM. For immunocytochemistry we recommend using cells fixed in 4% paraformaldehyde buffered with 50mM sodium borate at pH 9.5. Western blotting: Reacts with a band of 440 kD full length. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Concentration | 1.02 mg/ml |
| Buffer | This antibody is supplied as liquid, purified immunoglobulin fraction in PBS buffer pH 7.2 with 0.09% sodium azide as preservative. |
| Storage | Aliquote and store the antibody at–20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Restrictions | For Research Use only |
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