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Application
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Flow Cytometry (FACS)
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Application Notes
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This antibody is suitable for use in flow cytometry. This clone has also been reported to work in immunohistochemistry (both frozen and paraffin sections)(2). Strains reported to be positive for the 7/4 clone are: AKR, C57BL/6, C57BL/10, C58, DBA/2, MF1, NZB, NZW, SJL, Swiss (PO) and 129J. Strains reported to be negative/weak for the 7/4 clone are: A2G, A/Sn, ASW, BALB/c, C3H/HEH and CBA.T6T6. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
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Concentration
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0.2 mg/ml
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Buffer
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This antibody is supplied as liquid, purified Ig buffered in PBS and 0.09%
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Storage
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Store at 4°C. For long term storage, aliquot and freeze unused portion at -20°C in volumes appropriate for single usage. Avoid freeze/thaw cycles. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted.
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Research Area
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Immunology, Cell/Tissue Markers
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Restrictions
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For Research Use only
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