PARP (Human) antibody

Overview

Antigen	PARP
Reactivity	Human
Antibody Type	Polyclonal
Isotype	IgG, Ig »Matching secondary antibodies
Description	PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between Asp214 and Gly215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
Host	Rabbit
Specificity	The polyclonal anti-PARP antibody recognizes the full length and the cleaved fragment of human PARP. Not tested in other species.

Application	Western Blotting (WB)
Application Notes	The antibody can be used for Western blotting analysis (1 µg/ml). However, the optimal conditions should be determined individually.
Buffer	Supplied as 100 µg (0.2 mg/ml) affinity purified rabbit anti-PARP polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.
Research Area	Enzymes, Metabolism
Restrictions	For Research Use Only

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