PARP (Human) antibody

Overview

Antigen	PARP
Immunogen	Synthetic peptide, corresponding to amino acids 509-524 of Cow PARP (automodification domain), conjugated to a carrier.
Reactivity	Human, Mouse (Murine), Rat
Antibody Type	Polyclonal
Isotype	IgG »Matching secondary antibodies
Description	Poly(ADP-ribose) polymerase (PARP), a nuclear enzyme often termed a molecular nick sensor, recognizes and binds to DNA single or double strand breaks through the N-terminal DNA binding domain sensor. The activation of PARP is one of the first responses to DNA strand interruptions. The DNA-bound, catalytically activated PARP synthesizes poly(ADP-ribose)(pADPr) on nuclear target proteins such as topoisomerase, histones, and PARP itself. Although the exact role of PARP is not well known, it has been implicated in different cellular responses to genotoxic damage. These responses include cell survival and death, transformation, and DNA repair. Additional studies have demonstrated the role of PARP in the death of neuronal cells after ischemia-reperfusion injury, as well as maintaining the genomic integrity and survival responses of PARP knockout mice after exposure to whole body g-irradiation.
Host	Rabbit
Specificity	Recognizes 116 kDa PARP and the 85 kDa apoptosis-related cleavage fragment.

Application	ELISA, Western Blotting (WB)
Application Notes	For WB: Use at a dilution of 1:500. Not tested in other applications. Optimal dilutions/concenterations should be determined by the researcher.
Concentration	1 mg/ml
Purity	Whole antiserum
Buffer	Preservative: 0.05% Sodium AzideConstituents: 0.2% BSA, PBS
Storage	Keep as concentrated solution. Store at 4?C short term. For extended storage aliquot and store at -20?C or below. Avoid freeze-thaw cycles.
Restrictions	For research use only

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EMBL Harvester	Search human, mouse or rat genome for antigen PARP (Bioinformatic Harvester (EMBL))