Product details for  PARP antibody
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PARP antibody

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Image for PARP antibody
Antigen: PARP
Antibody Type: Monoclonal
Application: Immunofluorescence (IF), Western Blotting (WB)
Reactivity: Mouse (Murine), Human
Host: Mouse
Quantity: 25 µg (Product variants)
Price: 99,00 €   (Plus shipping costs and VAT)
Order number: ABIN160910
Availability: Ships within 7 to 10 days
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Additional Information:  PARP antibody

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Antigen PARP
Immunogen Recombinant (partial), N-terminal 2/3 sequence of PARP
Reactivity Mouse (Murine), Human
Antibody Type Monoclonal
Format Purified
Isotype Mouse IgG1, kappa »Matching secondary antibodies
Description PARP (Poly (ADP-ribose) polymerase) is a 113 kD nuclear protein that can exist as a homo- or hetero-dimer. This protein acts as a molecular nick sensor and functions in base excision repair, poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism and participates in protein modification to enhance or repress transcription. PARP is ribosylated by PARP2 and is a target for caspase cleavage during apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3. The 5A5 monoclonal antibody recognizes the N-terminal region of human and mouse PARP and has been shown to be useful for Western blotting and immunofluorescence staining. Distribution: Nuclear Function: Molecular nick sensor, base excision repair, catalyzes poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture, DNA metabolism, protein modification may enhance or repress transcription Modifications: Ribosylation by PARP2 Synonyms: Poly (ADP-ribose) polymerase Regulation: Poly(ADP-ribose) glycohydrolase removes ribose chains, allows quick, transient ribosylation of proteins Structure: PARP family, BRCT domain, homo- or hetero-dimer, 113 kD
Clone 5A5
Host Mouse
Specificity Component of a base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. Heterodimerizes with PARP2, interacts with PARP3, modifies TATA-BP, YY1, Sp1, NF-B, p53 and others.

Application Details:  PARP antibody

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Application Immunofluorescence (IF), Western Blotting (WB)
Application Notes Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Purification The antibody was purified by protein G affinity chromatography.
Buffer This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Storage Upon receipt, store undiluted at 4° C.
Research Area Enzymes, Metabolism
Restrictions For Research Use only

Publications:  PARP antibody

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Publications Cherney, McBride, Chen et al.:
"cDNA sequence, protein structure, and chromosomal location of the human gene for poly(ADP-ribose) polymerase."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 84, Issue 23, pp. 8370-4, 1988/01 (PubMed)

Ikejima, Noguchi, Yamashita et al.:
"The zinc fingers of human poly(ADP-ribose) polymerase are differentially required for the recognition of DNA breaks and nicks and the consequent enzyme activation. Other structures recognize intact DNA."
In: The Journal of biological chemistry , Vol. 265, Issue 35, pp. 21907-13, 1991/01 (PubMed)

Noel, Giocanti, Fernet et al.:
"Poly(ADP-ribose) polymerase (PARP-1) is not involved in DNA double-strand break recovery."
In: BMC cell biology , Vol. 4, pp. 7, 2004/01 (PubMed)

Ying, Sevigny, Chen et al.:
"Poly(ADP-ribose) glycohydrolase mediates oxidative and excitotoxic neuronal death."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 98, Issue 21, pp. 12227-32, 2001/01 (PubMed)

Images:  PARP antibody

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Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PARP antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system. Hela cells stained with PE-conjugated antibody against PARP Hela cells stained with DAPI to indicate nuclei Overlay of panels A and B, showing the nuclear localization of PARP

Image for PARP antibody (1)

Hela cells stained with PE-conjugated antibody against PARP Hela cells stained with DAPI to indicate nuclei Overlay of panels A and B, showing the nuclear localization of PARP

Image for PARP antibody (2)

Hela cells stained with DAPI to indicate nuclei Overlay of panels A and B, showing the nuclear localization of PARP

Image for PARP antibody (3)

External Information:  PARP antibody

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Google Scholar Find more references for clone 5A5 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen PARP (Bioinformatic Harvester (EMBL))