PARP antibody

Overview


	Antigen:	PARP
	Antibody Type:	Monoclonal
	Application:	Western Blotting (WB), Immunofluorescence (IF)
	Conjugate:	Biotin
	Reactivity:	Mouse (Murine), Human
	Host:	Mouse
	Quantity:	25 µg (Product variants)
	Price:	145,00 € (Plus shipping costs and VAT)
	Order number:	ABIN160912
Availability:	Ships within 7 to 10 days

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Additional Information: PARP antibody

Antigen	PARP
Immunogen	Recombinant (partial), N-terminal 2/3 sequence of PARP
Reactivity	Mouse (Murine), Human
Antibody Type	Monoclonal
Isotype	Mouse IgG1, kappa »Matching secondary antibodies
Description	PARP (Poly (ADP-ribose) polymerase) is a 113 kD nuclear protein that can exist as a homo- or hetero-dimer. This protein acts as a molecular nick sensor and functions in base excision repair, poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism and participates in protein modification to enhance or repress transcription. PARP is ribosylated by PARP2 and is a target for caspase cleavage during apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3. The 5A5 monoclonal antibody recognizes the N-terminal region of human and mouse PARP and has been shown to be useful for Western blotting and immunofluorescence staining. Distribution: Nuclear Function: Molecular nick sensor, base excision repair, catalyzes poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture, DNA metabolism, protein modification may enhance or repress transcription Modifications: Ribosylation by PARP2 Synonyms: Poly (ADP-ribose) polymerase Regulation: Poly(ADP-ribose) glycohydrolase removes ribose chains, allows quick, transient ribosylation of proteins Structure: PARP family, BRCT domain, homo- or hetero-dimer, 113 kD
Clone	5A5
Host	Mouse
Specificity	Component of a base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. Heterodimerizes with PARP2, interacts with PARP3, modifies TATA-BP, YY1, Sp1, NF-B, p53 and others.

Application Details: PARP antibody

Application	Western Blotting (WB), Immunofluorescence (IF)
Application Notes	Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Conjugate	Biotin
Purification	The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Buffer	This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Storage	The antibody solution should be stored undiluted at 4°C. Do not freeze.
Research Area	Enzymes, Metabolism
Restrictions	For Research Use only

Images: PARP antibody

Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PARP antibody. Proteins were visualized using a donkey anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system. Hela cells stained with PE-conjugated antibody against PARP Hela cells stained with DAPI to indicate nuclei Overlay of panels A and B, showing the nuclear localization of PARP

Image for PARP antibody (1)

Hela cells stained with PE-conjugated antibody against PARP Hela cells stained with DAPI to indicate nuclei Overlay of panels A and B, showing the nuclear localization of PARP

Image for PARP antibody (2)

Hela cells stained with DAPI to indicate nuclei Overlay of panels A and B, showing the nuclear localization of PARP

Image for PARP antibody (3)

Product variants

PARP antibody (Format: Purified, Quantity: 25 µg)

PARP antibody (Format: Purified, Quantity: 100 µg)

PARP antibody (Quantity: 100 µg, Conjugate: Biotin)

External Information: PARP antibody

Google Scholar	Find more references for clone 5A5 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen PARP (Bioinformatic Harvester (EMBL))