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Product details for PGP9.5 (Human) antibody
PGP9.5 (Human) antibody
Overview
| Antigen: | Protein Gene Product 9.5 (PGP9.5) |
| Antibody Type: | Monoclonal |
| Application: | Enzyme Immunoassay (EIA), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) |
| Reactivity: | Human, Rat, Guinea Pig |
| Host: | Mouse |
| Quantity: | |
| Price: | 350,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN110973 |
| Availability: | 3 to 10 days until shipment |
Additional Information: PGP9.5 (Human) antibody
| Antigen | Protein Gene Product 9.5 (PGP9.5) |
| Immunogen | Native PGP 9.5 from human brain |
| Reactivity | Human, Rat, Guinea Pig |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | IgG2a »Matching secondary antibodies |
| Clone | 13C4 |
| Host | Mouse |
| Specificity | This antibody stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibers in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumors of the DNES. It also stains neuroendocrine cells in human adult gut (unlike clone 31A3). Clones 31A3 and 13C4 each recognise a different epitope towards the N-terminus of the protein. Clone 13C4 reacts with PGP 9.5 from human, rat and guinea pig. |
Application Details: PGP9.5 (Human) antibody
| Application | Enzyme Immunoassay (EIA), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) |
| Application Notes | Immunohistochemistry on frozen and paraffin embedded tissues (dilute 1:50-1:400, The antigen is stable in formalin fixed paraffin embedded sections, however we recommend fixation in 95% ethanol/5% acetic acid for 2-3 hours prior to paraffin embedding. This fixation can replaced by standard methods if the sections are subjected to microwave pre-treatment in citrate buffer). ELISA (EPT: 1/160K in a simple ELISA using native protein coated at 100 ng/well.). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Concentration | 5.0 mg/ml Clone No.: 13C4 |
| Buffer | This antibody is supplied as liquid, Protein A affinity purified immunoglobulin fraction in PBS pH 7.2 containing 0.09% sodium azide as preservative. |
| Storage | Store the antibody at –20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Research Area | Tags/Labels |
| Restrictions | For Research Use only |
External Information: PGP9.5 (Human) antibody
| Google Scholar | Find more references for clone 13C4 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Protein Gene Product 9.5 (PGP9.5) (Bioinformatic Harvester (EMBL)) |
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