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Product details for PLK-1 Human antibody
PLK-1 Human antibody
Overview
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| Antigen: | PLK-1 |
| Antibody Type: | Monoclonal |
| Application: | Western Blotting (WB), Immunoprecipitation (IP) |
| Reactivity: | Human, Mouse (Murine) |
| Host: | Mouse |
| Quantity: | |
| Price: | 226,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN161106 |
| Availability: | Ships within 7 to 10 days |
Additional Information: PLK-1 Human antibody
| Antigen | PLK-1 |
| Immunogen | Amino Acid: 300-603 of human PLK-1 |
| Reactivity | Human, Mouse (Murine) |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | Mouse IgG1, kappa »Matching secondary antibodies |
| Description | PLK-1 (polo-like kinase 1) is a member of te serine/threonine protein kinase family, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila), PLK-1 contains two polo box domains with a predicted molecular weight of 68 kD. This nuclear protein is highly expressed in placenta and colon and has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK-1 may also be required for cell division, depletion of PLK-1 results in apoptosis. PLK-1 is upregulated by growth stimulating agents and is regulated by cell cycle position (highest in G2/M phase, declining to nearly undetectable levels after mitosis and throughout G1). PLK-1 is modified by phosphorylation (Thr210 is the major phosphorylation site in activated PLK-1 from mitotic cells) and has been shown to interact with nuclear distribution gene C. The 3F8 monoclonal antibody recognizes human and mouse PLK-1 and has been shown to be useful for Western blotting. Distribution: Nuclear protein, highly expressed in placenta and colon Function: Regulates cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. May be required for cell division. Depletion of PLK-1 results in apoptosis Modifications: Phosphorylation Synonyms: Serine/Threonine protein kinase PLK, Polo-like kinase (PLK), Serine-threonine protein kinase 13 Regulation: Upregulated by growth stimulating agents. Regulated by cell cycle position (highest in G2/M phase and declines to nearly undetectable levels after mitosis and throughout G1) Structure: Serine/Threonine family of protein kinases, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila). Contains two polo box domains. Predicted molecular weight 68 kD |
| Clone | 3F8 |
| Host | Mouse |
| Specificity | Interacts with nuclear distribution gene C. |
Application Details: PLK-1 Human antibody
| Application | Western Blotting (WB), Immunoprecipitation (IP) |
| Application Notes | Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application. |
| Purification | The antibody was purified by affinity chromatography. |
| Buffer | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml. |
| Storage | The antibody solution should be stored undiluted at 4 °C. |
| Restrictions | For Research Use only |
Images: PLK-1 Human antibody
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Hela cell extract (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Plk-1 (Clone 3F8) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. |
Product variants
| PLK-1 Human antibody (Format: Purified, Quantity: 25 µg) |
| PLK-1 Human antibody (Quantity: 25 µg, Conjugate: Biotin) |
| PLK-1 Human antibody (Quantity: 100 µg, Conjugate: Biotin) |
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