BASKET
HELP
Product details for Phosphotyrosine (Dog (Canine)) antibody
Phosphotyrosine (Dog (Canine)) antibody
Overview
| Antigen: | Phosphotyrosine |
| Antibody Type: | Monoclonal |
| Application: | Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB) |
| Reactivity: | Dog (Canine), Human, Mouse (Murine), Rat |
| Host: | Mouse |
| Quantity: | |
| Price: | 280,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN126875 |
| Availability: | 3 to 10 days until shipment |
Additional Information: Phosphotyrosine (Dog (Canine)) antibody
| Antigen | Phosphotyrosine |
| Immunogen | Synthetic phosphopeptide conjugated to KLH |
| Reactivity | Dog (Canine), Human, Mouse (Murine), Rat |
| Antibody Type | Monoclonal |
| Format | Purified |
| Isotype | IgG1 »Matching secondary antibodies |
| Clone | 3B12 |
| Host | Mouse |
| Specificity | Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains. ABIN126875 recognizes phosphotyrosine in the context of the surrounding amino acids, showing high preference for phosphorylated EGF receptor. It reacts with human, mouse, rat and dog. |
Application Details: Phosphotyrosine (Dog (Canine)) antibody
| Application | Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB) |
| Application Notes | Suitable for Immunoprecipitation (1 - 10 µg per 106 pervanadate-treated A431 cells, use phosphotyrosine MW standardas positive control), Western Blot (0.5 µg/ml for HRPO/ECL detection, recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% (v/v) Tween 20, 2x PBS) and ELISA (0.05 µg/ml). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Buffer | This antibody is lyophilized from 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose. It was purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography. Reconstitute with 1 ml H2O (15 min, RT). |
| Storage | For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freeze / thaw cycles! |
| Research Area | Amino Acids, Protein Modifications |
| Restrictions | For Research Use only |
External Information: Phosphotyrosine (Dog (Canine)) antibody
| Google Scholar | Find more references for clone 3B12 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Phosphotyrosine (Bioinformatic Harvester (EMBL)) |
browse antibodies by
Antigens
Secondary Antibodies
Research areas
Clones
Hosts
Applications
Reactivity
Supplier
Antigens
Secondary Antibodies
Research areas
Clones
Hosts
Applications
Reactivity
Supplier
