Selenium Binding Protein (Human) antibody

Overview


	Antigen:	Selenium Binding Protein
	Antibody Type:	Monoclonal
	Application:	Western Blotting (WB), Immunohistochemistry (IHC)
	Reactivity:	Human, Mink, Mouse (Murine)
	Host:	Mouse
	Quantity:	100 µg
	Price:	251,00 € (Plus shipping costs and VAT)
	Order number:	ABIN131897
	Availability:	7 to 10 days until shipment

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Additional Information: Selenium Binding Protein (Human) antibody

Antigen	Selenium Binding Protein
Immunogen	This antibody was purified from mouse ascites fluid using protein-A Sepharose. This hybridoma was established by fusion of mouse myeloma cell SP2/0 with Balb/c splenocyte immunized with GST-full length human SBP fusion protein.
Reactivity	Human, Mink, Mouse (Murine)
Antibody Type	Monoclonal
Format	Lyophilised
Isotype	IgG1., IgG1 »Matching secondary antibodies
Description	Selenium is an essential trace element that has confers tolerance to the toxic manifestation of various heavy metal exposures (for example, cadmium, mercury, lead and arsenic etc.) and chemical substances including free radicals. Selenium supplementation within the range of normal dietary intake can significantly decrease the incidence and mortality of several cancers. Moreover, it is reported that selenium deficiency may cause certain neurological diseases including schizophrenia. Selenium is thought to be mobilized by selenoproteins that accept the selenium as selenocysteine and proteins that bind selenium ion(s) after synthesis. Murine selenium binding protein (mouse SBP, mSP56) is one of the proteins that bind selenium. It is a 56 kDa cytosolic protein whose physiological properties are largely unknown. Mouse SBP is extremely homologous to another 56kDa mouse protein named acetaminophen binding protein (mouse AcBP), which binds to a metabolite of acetaminophen that can cause serious liver injury, suggesting that both 56kDa proteins may be involved in detoxification processes. Human 56kDa selenium binding protein (human SBP) is a protein that has higher homology to 56kDa mouse SBP rather than to mouse AcBP. Human SBP is expressed in liver, lung and kidney at high levels and in heart and intestine at lower levels.
Clone	4D4
Host	Mouse
Specificity	This antibody detects human SBP migrated to ~55kDa position on Western blotting with total cell lysate from human cell line, for example, KB, HeLa. Species cross reactivity has also been tested. Recognizes SBP in human HeLa and KB cell lines, and monkey COS cells. SBP was not detected in human Jurkat, Raji, or HL-60 cell lines. This antibody may cross react weakly with mouse SBP in WR19L and Ba/F3 cell lines. The antibody was negative for rat SBP using 3Y1-B cells

Application Details: Selenium Binding Protein (Human) antibody

Application	Western Blotting (WB), Immunohistochemistry (IHC)
Application Notes	Western blotting: 1 µg / mL for chemiluminescence detection system. Immunoprecipitation: Not tested. Immunohistochemistry: 2 µg / mL
Buffer	This antibody is lyophilized form. Prepare a stock solution by dissolving it with 100 µL distilled water to make 1.0 mg/mL IgG in PBS (pH7.2) containing 1% sucrose. Contains no preservatives.
Restrictions	For Research Use Only

Publications: Selenium Binding Protein (Human) antibody

Publications

Bansal, Oborn, Danielson et al.:
"Evidence for two selenium-binding proteins distinct from glutathione peroxidase in mouse liver."
In: Carcinogenesis , Vol. 10, Issue 3, pp. 541-6, 1989/01 (PubMed)

Bansal, Mukhopadhyay, Scott et al.:
"DNA sequencing of a mouse liver protein that binds selenium: implications for selenium's mechanism of action in cancer prevention."
In: Carcinogenesis , Vol. 11, Issue 11, pp. 2071-3, 1990/01 (PubMed)

Chang, Tsui, Liew et al.:
"Isolation, characterization, and chromosomal mapping of a novel cDNA clone encoding human selenium binding protein."
In: Journal of cellular biochemistry , Vol. 64, Issue 2, pp. 217-24, 1997/01 (PubMed)

Lanfear, Fleming, Walker et al.:
"Different patterns of regulation of the genes encoding the closely related 56 kDa selenium- and acetaminophen-binding proteins in normal tissues and during carcinogenesis."
In: Carcinogenesis , Vol. 14, Issue 3, pp. 335-40, 1993/01 (PubMed)

Pumford, Martin, Hinson:
"A metabolite of acetaminophen covalently binds to the 56 kDa selenium binding protein."
In: Biochemical and biophysical research communications , Vol. 182, Issue 3, pp. 1348-55, 1992/01 (PubMed)

Images: Selenium Binding Protein (Human) antibody

Paraffin section of well-differentiated human primary lung adenocarcinoma using anti-SBP at 1:500 (2 ?g/ml).

Image for Selenium Binding Protein (Human) antibody (1)

External Information: Selenium Binding Protein (Human) antibody

Google Scholar	Find more references for clone 4D4 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Selenium Binding Protein (Bioinformatic Harvester (EMBL))