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Product details for Serotonin Transporter (SERT) (Human) antibody
Serotonin Transporter (SERT) (Human) antibody
Overview
| Antigen: | Serotonin Transporter (SERT) |
| Antibody Type: | Polyclonal |
| Application: | Enzyme Immunoassay (EIA), Western Blotting (WB) |
| Reactivity: | Human, Rat, Mouse (Murine) |
| Host: | Rabbit |
| Quantity: | |
| Price: | 380,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN154861 |
| Availability: | Ships in 7 to 10 days |
Additional Information: Serotonin Transporter (SERT) (Human) antibody
| Antigen | Serotonin Transporter (SERT) |
| Reactivity | Human, Rat, Mouse (Murine) |
| Antibody Type | Polyclonal |
| Format | Aff - Purified |
| Host | Rabbit |
| Specificity | The rat SERT11 peptide sequence is 100% homologous in human and mouse. The antibodies have been shown crossreact with rat and human (2). |
Application Details: Serotonin Transporter (SERT) (Human) antibody
| Application | Enzyme Immunoassay (EIA), Western Blotting (WB) |
| Application Notes | Western Blotting (1:1K-5K for neat serum and 1-10 µg/ml for affinity pure using Chemiluminescence technique). An antibody raised against a similar peptide has been shown to react with a SERT in baculovirus cells expressing recombinant rat and human-SERT (2). Antibody activity may be reduced considerably when detecting endogenous SERT from rat or human cells. SERT has been observed to migrate as 94 kDa (rat platelet), 80 kDa (pulmonary membranes, and 76 kDa (rat brain) (2). This could be due to differences in postranslational modifications (e.g., glycosylation). ELISA (1:100K, using 50-100 ng SERT control peptide/well). Immunoprecipitations . An antibody to the SERT epitope has been shown to be effective in immunoprecipitating the 61 kDa species from rat and human SERT-transfected HeLa cells (2). Histochemistry & Immunofluorescence: Not tested. We recommend the testing of affinity purified antibody at 10-50 µg/ml on frozen sections of tissues fixed with 4% paraformaldehyde. |
| Buffer | Control peptide Solution is provided in PBS, pH 7.4 at 1 mg/ml (100 µg/100 µl). Antiserum is supplied as neat serum (100 µl soln or lyophilized). Affinity pure antibodies were purified over the peptide-Sepharose column and supplied as 1 mg/ml soln in PBS, |
| Research Area | Hormones, Transporters |
| Restrictions | For Research Use only |
External Information: Serotonin Transporter (SERT) (Human) antibody
| Google Scholar | Find more references for antigen Serotonin Transporter (SERT) on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Serotonin Transporter (SERT) (Bioinformatic Harvester (EMBL)) |
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