Streptococcus Group A FITC antibody

Overview

Antigen	Streptococcus Group A
Immunogen	Streptococci, Group A
Antibody Type	Polyclonal
Host	Rabbit
Specificity	Does not react with other Strep groups. No immunological cross reactivity. However, there is potential non-specific reactivity with such organisms as group C Streptococcus and Staphyloccus due to the presence of protein A like proteins (Staphyloccus) and protein C like proteins (Streptococcus C) present on the intact surface of these bacteria. These proteins bind any immunoglobulin (including the FITC Conjugate) and therefore will appear to fluoresce (non-specifically)

Application	Immunofluorescence (IF)
Application Notes	Direct Fluorescenceassay staining of target antigens in a permissive tissue culture system. Working dilution must be determined by the user for his or her application but a starting range of 1:10-1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. Pre-block slide with 10% normal rabbit serum. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Conjugate	FITC
Concentration	1.0mg/ml (A280nm)
Buffer	This antibody is supplied as liquid, purified Ig fraction, covalently coupled with high purity Isomer I of fluorescein isothiocyanate (Care is taken to ensure complete removal of any free fluorescein from the final product) in 0.01 M PBS pH 7.2 containing
Storage	Short term (up to 6 months) store at 2-8°C under subdued light. Long term, aliquot and store at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area	Bacteria
Restrictions	For Research Use only

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