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Product details for Syntaxin-7 antibody
Syntaxin-7 antibody
Overview
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| Antigen: | Syntaxin-7 |
| Antibody Type: | Monoclonal |
| Application: | Immunohistochemistry (IHC) |
| Host: | Mouse |
| Quantity: | |
| Price: | 216,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN131693 |
| Availability: | 7 to 10 days until shipment |
Additional Information: Syntaxin-7 antibody
| Antigen | Syntaxin-7 |
| Immunogen | This antibody was purified from hybridoma (clone 1C3) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3653-NS-1 with Balb/c mouse splenocyte immunized with a human recombinant syntaxin-7 (a.a. 2-242). |
| Antibody Type | Monoclonal |
| Isotype | IgG1 »Matching secondary antibodies |
| Description | Syntaxin 7, a Vps6p homologue, is a soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) that mediates endocytic trafficking. Syntaxin 7 is localized to membranes of late endosome compartments, where it is required for the fusion of late endosomes and lysosomes. Levels of syntaxin 7 are elevated in melanomas and formation of complexes with syntaxin-7, syntaxin-8, endobrevin/VAMP 8, and vti1b may be involved in fusion events leading to melanogenesis. |
| Clone | 1C3 |
| Host | Mouse |
| Specificity | This antibody reacts with syntaxin-7 (38-40 kDa). |
Application Details: Syntaxin-7 antibody
| Application | Immunohistochemistry (IHC) |
| Application Notes | Immunohistochemical staining for paraffin-embedded sections: SAB method 1) Deparaffinize the sections with Xylene 3 times for 3-5 minutes each. 2) Wash the slides with Ethanol 3 times for 3-5 minutes each. 3) Wash the slides with PBS 3 times for 3-5 minutes each. 4) Heat treatment Heat treatment by microwave oven:Place the slides put on staining basket in 500 mL beaker with 500 mL citrate buffer (pH 6.5). Cover the beaker with plastic wrap, then process the slides 2 times for 10 minutes each at 500 W with microwave oven. Let the slides cool down in the beaker at room temperature for about 40 minutes. 5) Remove the slides from the citrate buffer and cover each section with 3% H 2 O 2 for 10 minutes at room temperature to block endogenous peroxidase activity. Wash 3 times in PBS for 5 minutes each. 6) Remove the slides from PBS, wipe gently around each section and cover tissues with Protein Blocking Agent (Ultratech HRP Kit, IMMUNOTECH, code no. IM-2391) for 5 minutes to block non-specific antibody staining. Do not wash. 7) Tip off the blocking buffer, wipe gently around each section and cover tissues with primary antibody diluted with PBS containing 1% BSA as suggested in the Applications. 8) Incubate the sections for 1 hour at room temperature. 9) Wash the slides 3 times in PBS for 5 minutes each. 10) Wipe gently around each section and cover tissues with Polyvalent Biotinylated Antibody (Ultratech HRP Kit). Incubate for 10 minutes at room temperature. Wash as in step 9. 11) Wipe gently around each section and cover tissues with Streptavidin-Peroxidase (Ultratech HRP Kit). Incubate for 10 minutes at room temperature. Wash as in step 9. 12) Visualize by reacting for 10-20 minutes with substrate solution containing 7.5 mg DAB, 40 µL of 30% H 2 O 2 in 150 mL PBS. * DAB is a suspected carcinogen and must be handled with care. Always wear gloves. 13) Wash the slides in water for 5 minutes. 14) Counter stain in hematoxylin for 1 minute, wash the slides 3 times in water for 5 minutes each, and then immerse the slides in PBS for 5 minutes. Dehydrate by immersing in Ethanol 3 times for 3 minutes each, followed by immersing in Xylene 3 times for 3 minutes each. 15) Now ready for mounting. Positive control for Immunohistochemistry: human kidney 031025-1 Western blotting: Not recommended. This antibody detects recombinant GST-syntaxin-7, but do not detect sytaxin-7 in any cell lines or brain lysates. Immunoprecipitation: Not recommended. Immunohistochemistry: 1-5 µg / mL Heat treatment is necessary for paraffin embedded sections. Microwave oven: 2 times for 10 minutes each in citrate buffer (pH 6.5). Immunocytochemistry: Not tested. Flow Cytometry: Not tested. |
| Concentration | 1 mg/mL |
| Buffer | 100 µg IgG in 100 µL volume of PBS containing 50% glycerol, pH 7.2. Contains no preservatives. |
| Restrictions | For Research Use Only |
Publications: Syntaxin-7 antibody
| Publications |
Mullock, Smith, Ihrke et al.: "Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and Is required for late endosome-lysosome fusion." In: Molecular biology of the cell , Vol. 11, Issue 9, pp. 3137-53, 2000/01 (PubMed) Wade, Bryant, Connolly et al.: "Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells." In: The Journal of biological chemistry , Vol. 276, Issue 23, pp. 19820-7, 2001/01 (PubMed) |
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