TLR6 (Human) antibody

Overview


	Antigen:	Toll-like Receptor 6 (TLR6)
	Antibody Type:	Monoclonal
	Application:	Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
	Reactivity:	Human
	Host:	Mouse
	Quantity:	0.1 mg (0.5 mg/ml)
	Price:	280,00 € (Plus shipping costs and VAT)
	Order number:	ABIN121322
	Availability:	Ships in 3 to 10 days

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Additional Information: TLR6 (Human) antibody

Antigen	Toll-like Receptor 6 (TLR6)
Immunogen	Synthetic peptide corresponding to amino acids 408-424 of human TRL6
Reactivity	Human
Antibody Type	Monoclonal
Format	Purified
Isotype	IgG1 »Matching secondary antibodies
Clone	86B1153
Host	Mouse
Specificity	The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. The amino acid sequence of human TLR6 is most similar to hTLR1 with 69% identity at the amino acid level. Human TLR6 consists of a predicted 807 amino acids with a molecular weight of approximately 92 kDa (3). Human and mouse TLR6 share an amino acid identity of 73%. TLR6 activates both NF-kB and c-Jun N-terminal Kinase (JNK). The amino acids in the cytoplasmic domain of IL-1 receptor, which are critical for NF-kB activation, are conserved in TLR6. It has also been shown that TLR2-mediated response to a phenol-soluble factor from staphylococcus epidermidis is enhanced by TLR6 (4). TLR6 is predominantly expressed in spleen, thymus, ovary, and lung (3). This antibody is specific for human TLR6 and will not cross-react with mouse TLR6 or other TLR´s.

Application Details: TLR6 (Human) antibody

Application	Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
Application Notes	Flow cytometry analysis at 3-5 ug/1,000,000 cells. Ramos cells can be used as a positive control. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration	0.5 mg/ml
Buffer	This antibody is supplied as liquid IgG purified on Protein G in PBS, pH 7.2, with 0.1% sodium azide. Intracellular FACS analysis of TLR6 (shaded peak) and mouse IgG1 isotype control (open peak) in 106 Ramos cells using 3 ug of ABIN121322
Storage	Store the antibody at 4-8°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area	Signaling, Immunology, Inflammation
Restrictions	Research only

Publications: TLR6 (Human) antibody

Publications

Hajjar, OMahony, Ozinsky et al.:
"Cutting edge: functional interactions between toll-like receptor (TLR) 2 and TLR1 or TLR6 in response to phenol-soluble modulin."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 166, Issue 1, pp. 15-9, 2001/01 (PubMed)

Muzio, Natoli, Saccani et al.:
"The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6)."
In: The Journal of experimental medicine , Vol. 187, Issue 12, pp. 2097-101, 1998/01 (PubMed)

Takeuchi, Kawai, Sanjo et al.:
"TLR6: A novel member of an expanding toll-like receptor family."
In: Gene , Vol. 231, Issue 1-2, pp. 59-65, 1999/01 (PubMed)

Medzhitov, Janeway:
"Innate immunity: the virtues of a nonclonal system of recognition."
In: Cell , Vol. 91, Issue 3, pp. 295-8, 1997/01 (PubMed)

Images: TLR6 (Human) antibody

Intracellular FACS analysis of TLR6 (shaded peak) and mouse IgG1 isotype control (open peak) in 106 Ramos cells using 3 ug of ABIN121322

Image for TLR6 (Human) antibody (1)

External Information: TLR6 (Human) antibody

Google Scholar	Find more references for clone 86B1153 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Toll-like Receptor 6 (TLR6) (Bioinformatic Harvester (EMBL))