TLR6 (Mouse (Murine)) antibody

Overview


	Antigen:	Toll-like Receptor 6 (TLR6)
	Antibody Type:	Polyclonal
	Application:	Flow Cytometry (FACS), Western Blotting (WB)
	Reactivity:	Mouse (Murine), Human
	Host:	Rabbit
	Quantity:	0.1 mg (0.5 mg/ml)
	Price:	300,00 € (Plus shipping costs and VAT)
	Order number:	ABIN123392
	Availability:	Ships in 3 to 10 days

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Additional Information: TLR6 (Mouse (Murine)) antibody

Antigen	Toll-like Receptor 6 (TLR6)
Immunogen	Synthetic peptide of mouse TRL6
Reactivity	Mouse (Murine), Human
Antibody Type	Polyclonal
Format	Purified
Host	Rabbit
Specificity	The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. The amino acid sequence of human TLR6 is most similar to hTLR1 with 69% identity at the amino acid level. Human TLR6 consists of a predicted 807 amino acids with a molecular weight of approximately 92 kDa (3). Human and mouse TLR6 share an amino acid identity of 73%. TLR6 activates both NF-kB and c-Jun N-terminal Kinase (JNK). The amino acids in the cytoplasmic domain of IL-1 receptor, which are critical for NF-kB activation, are conserved in TLR6. It has also been shown that TLR2-mediated response to a phenol-soluble factor from staphylococcus epidermidis is enhanced by TLR6 (4). TLR6 is predominantly expressed in spleen, thymus, ovary, and lung (3). This antibody recognizes mouse TLR6 and will cross-react with human TLR6. Western blot analysis of mTLR6 in 15 ug of Raw cell lysate using ABIN123392 at 1 ug/ml

Application Details: TLR6 (Mouse (Murine)) antibody

Application	Flow Cytometry (FACS), Western Blotting (WB)
Application Notes	Western blot (1-2 ug/ml, A specific 92 kD band is detected. Raw or Ramos cells can be used as a positive control). Flow cytometry (2 ug /1x106 cells). Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration	0.5 mg/ml
Buffer	This antibody is supplied as liquid IgG purified on Protein G in PBS, pH 7.2, with 0.1 mg/ml BSA and 0.02% sodium azide.
Storage	Store the antibody at 4-8°C for one month or at –20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Research Area	Signaling, Immunology, Inflammation
Restrictions	Research only

Publications: TLR6 (Mouse (Murine)) antibody

Publications

Hajjar, OMahony, Ozinsky et al.:
"Cutting edge: functional interactions between toll-like receptor (TLR) 2 and TLR1 or TLR6 in response to phenol-soluble modulin."
In: Journal of immunology (Baltimore, Md. : 1950) , Vol. 166, Issue 1, pp. 15-9, 2001/01 (PubMed)

Medzhitov, Janeway:
"Innate immunity: the virtues of a nonclonal system of recognition."
In: Cell , Vol. 91, Issue 3, pp. 295-8, 1997/01 (PubMed)

Muzio, Natoli, Saccani et al.:
"The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6)."
In: The Journal of experimental medicine , Vol. 187, Issue 12, pp. 2097-101, 1998/01 (PubMed)

Takeuchi, Kawai, Sanjo et al.:
"TLR6: A novel member of an expanding toll-like receptor family."
In: Gene , Vol. 231, Issue 1-2, pp. 59-65, 1999/01 (PubMed)

Images: TLR6 (Mouse (Murine)) antibody

Flow analysis of TLR6 in RAW cells using 2 ug of ABIN123392. Shaded histogram represents RAW cells without anti-TLR6 antibody, green represents isotype control, purple represents anti-TLR6 antibody.

Image for TLR6 (Mouse (Murine)) antibody (1)

Western blot analysis of mTLR6 in 15 ug of Raw cell lysate using ABIN123392 at 1 ug/ml

Image for TLR6 (Mouse (Murine)) antibody (2)

External Information: TLR6 (Mouse (Murine)) antibody

Google Scholar	Find more references for antigen Toll-like Receptor 6 (TLR6) on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Toll-like Receptor 6 (TLR6) (Bioinformatic Harvester (EMBL))