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Applikation
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Immunohistochemistry ((Frozen Sections) (IHC) (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Applikationshinweise
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This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:10 to 1:25 with AutoProbe III. When using routinely processed tissues, a high temperature antigenic unmasking technique (see instructions below) is recommended for uniform and reproducible results. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.
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Buffer
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20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, and 0.05% Sodium Azide, pH 7.5.
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Lagerung
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Store at 4 C. Do not freeze.
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Forschungsgebiet
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Immunology
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Beschränkungen
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For research use only
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