Thioredoxin [2C9] antibody

Overview

Antigen	Thioredoxin [2C9]
Immunogen	: This antibody was purified from mouse ascites fluid using protein-A Sepharose. This hybridoma was established by fusion of mouse myeloma P3U1 with Balb/c splenocyte immunized with E. coli thioredoxin (trxA) (109 a.a.). Product
Reactivity	E. Coli
Antibody Type	Monoclonal
Format	Liquid
Isotype	IgG1k »Matching secondary antibodies
Description	The thioredoxin (Trx) fusion E. coli expression system is available to offer soluble expression of normally insoluble or difficult to express proteins. LaVallie et al.1) reported that a number of mammalian cytokines and growth factors, when expressed as C-terminal trxA fusion proteins, stayed remarkably soluble in the E. coli cytoplasm under certain conditions.
Clone	2C9
Host	Mouse

Application	Western Blotting (WB)
Application Notes	SDS PAGE & Western Blotting 1) Boil all samples for 3~5 minutes. Load 10 µ l of cell lysate or tissue homogenate (5~20 µ g total protein) to each well of an SDS-polyacrylamide gel and electrophorese in a 1 mm thick gel. 2) Transfer to a polyvinylidene difluoride (PVDF) membrane at 10V for 1 hour in a semi-dry transfer system. (Transfer Western blotting: 1:1000 for chemiluminescence detection system West-Western blotting: can be used. Details are provided concerning the following research applications. Research Use: F or research use only. Not for use in in vitro diagnostic procedures for clinical diagnosis. REFERENCE:1) LaVallie E. R. et al. Bio/Technology 11, 187 (1993) 2) Novy R. et al. inNoVAtions 3 , 7 (1995)
Buffer	25mM Tris, 190mM glycine, 20% MeOH). 3) The transferred proteins can be visualized by staining the membrane for 1 minute with Ponceau S (SIGMA Cat. No. P-7170). Rinse the membrane with PBS. 4) Non-specific binding sites are blocked by immersing the membra
Research Area	Coagulation, Enzymes, Metabolism
Restrictions	For Research Use Only

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