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Product details for Thioredoxin (Trx) (E. coli) antibody
Thioredoxin (Trx) (E. coli) antibody
Overview
| Antigen: | Thioredoxin (Trx) |
| Antibody Type: | Monoclonal |
| Application: | Western Blotting (WB) |
| Reactivity: | E. Coli |
| Host: | Mouse |
| Quantity: | |
| Price: | 251,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN131836 |
| Availability: | 7 to 10 days until shipment |
Additional Information: Thioredoxin (Trx) (E. coli) antibody
| Antigen | Thioredoxin (Trx) |
| Immunogen | : This antibody was purified from mouse ascites fluid using protein-A Sepharose. This hybridoma was established by fusion of mouse myeloma P3U1 with Balb/c splenocyte immunized with E. coli thioredoxin (trxA) (109 a.a.). Product |
| Reactivity | E. Coli |
| Antibody Type | Monoclonal |
| Format | Liquid |
| Isotype | IgG1k »Matching secondary antibodies |
| Description | The thioredoxin (Trx) fusion E. coli expression system is available to offer soluble expression of normally insoluble or difficult to express proteins. LaVallie et al.1) reported that a number of mammalian cytokines and growth factors, when expressed as C-terminal trxA fusion proteins, stayed remarkably soluble in the E. coli cytoplasm under certain conditions. |
| Clone | 2C9 |
| Host | Mouse |
Application Details: Thioredoxin (Trx) (E. coli) antibody
| Application | Western Blotting (WB) |
| Application Notes | SDS PAGE & Western Blotting 1) Boil all samples for 3~5 minutes. Load 10 µ l of cell lysate or tissue homogenate (5~20 µ g total protein) to each well of an SDS-polyacrylamide gel and electrophorese in a 1 mm thick gel. 2) Transfer to a polyvinylidene difluoride (PVDF) membrane at 10V for 1 hour in a semi-dry transfer system. (Transfer Western blotting: 1:1000 for chemiluminescence detection system West-Western blotting: can be used. Details are provided concerning the following research applications. Research Use: F or research use only. Not for use in in vitro diagnostic procedures for clinical diagnosis. REFERENCE:1) LaVallie E. R. et al. Bio/Technology 11, 187 (1993) 2) Novy R. et al. inNoVAtions 3 , 7 (1995) |
| Buffer | 25mM Tris, 190mM glycine, 20% MeOH). 3) The transferred proteins can be visualized by staining the membrane for 1 minute with Ponceau S (SIGMA Cat. No. P-7170). Rinse the membrane with PBS. 4) Non-specific binding sites are blocked by immersing the membra |
| Research Area | Coagulation, Enzymes, Metabolism |
| Restrictions | For Research Use Only |
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