购物车
帮助
抗原
产品详细信息： Thymine Dimer antibody
Thymine Dimer antibody
概述
| 抗原: | Thymine Dimer |
| 抗体类型: | 单克隆抗体 |
| 应用范围: | Blocking Antibody, Dot Blot (Dot), Southern Blotting (SB), Western Blotting (WB) |
| 抗体来源: | Mouse |
| 规格: | |
| 价格: | 247,00 € (加上运费和增值税) |
| 货号: | ABIN145119 |
| 发货时间: | 7 to 10 days until shipment |
详细信息: Thymine Dimer antibody
| 抗原 | Thymine Dimer |
| 免疫原 | Chemical / Small Molecule. |
| 抗体类型 | 单克隆抗体 |
| 状态 | Liquid |
| 抗体同种型 | IgG1 »相配的二抗 |
| 描述 | Non-radioactive labeling of DNA is typically based on the enzymatic incorporation of modified nucleotides, carrying a small chemical moiety such as biotin, digoxigenin or fluorescein. These tags are subsequently detected by specific reagents such as streptavidin or a specific antibody coupled to a signal-producing enzyme. Although very efficient and reliable, labeling by in vitro polymerization is time-consuming, expensive, and may require various post-label purification steps to remove an excess of unincorporated precursors. An alternative strategy for DNA labeling, is based on the UV-induced formation of cyclobutane thymine dimers. Several methods have been described for the detection of thymine dimers, which are based on chromato-graphic analysis, and on biochemical analysis with endonucleases specific for UV-irradiated DNA. In addition, methods utilizing antibodies specific for pyrimidine dimers and other UV-induced DNA lesions have evolved, which permit the study of the induct |
| 克隆 | H3 |
| 抗体来源 | Mouse |
| 特异性 | Monoclonal Anti-Thymine Dimer reacts specifically with the (5?-6?) cyclobutane type of homothymine or thymine-cytosine heterodimers. The antibody reacts with thymine dimers in single-stranded DNA, and has a lower affinity for the dimer in short oligonucle |
使用细节: Thymine Dimer antibody
| 应用范围 | Blocking Antibody, Dot Blot (Dot), Southern Blotting (SB), Western Blotting (WB) |
| 应用备注 | SB: Use at a concentration of 0.5-1.0 ug/ml. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| 浓度 | 2 mg/ml |
| 纯度 | Protein G purified |
| 缓冲液 | Preservative: 15mM Sodium AzideConstituents: PBS. pH 7.4 |
| 限制 | For research use only |
