Tyrosine antibody

Overview

Antigen:	Tyrosine
Antibody Type:	Polyclonal
Host:	Rabbit
Quantity:	100 µl
Price:	379,50 € (Plus shipping costs and VAT)
Order number:	ABIN107991
Availability:	7 days until shipment

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Additional Information: Tyrosine antibody

Antigen	Tyrosine
Immunogen	Synthetic L.Tyrosine conjugated to protein carrier (PC)
Antibody Type	Polyclonal
Format	Lyophilized
Isotype	IgG »Matching secondary antibodies
Host	Rabbit
Specificity	Conjugated L.Tyrosine . Using a conjugate L.Tyrosine-PC, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Compounds Cross-reactivity ratio (a) L.Tyrosine-G-PC 1 L.Phenylalanine-G-PC 1/6,000 L.Tryptophan-G-PC 1/35,000 L.Tyrosine-GA-PC 1/>50,000 L.Tyrosine (free) 1/>50,000 (a): L.Tyrosine-G-PC concentration / unconjugated or conjugated catecholamine concentration at half displacement G = Glutaraldehyde, GA = Glutaric anhydride

Application Details: Tyrosine antibody

Application Notes	Example of Immunocytochemistry application Detection of conjugated L.Tyrosine in rat brain 1- Perfusion: The rat is anaesthetized with sodium Pentobarbital or Nembutal and perfused intracardially through the aorta using a pump with the following solutions: Solution A: cacodylate 0.1M, sodium metabisulfite 10g/l, pH = 6.2 Solution B: cacodylate 0.1M, sodium metabisulfite 10g/l and glutaraldehyde 3-5%, pH = 7.5 2- Post fixation: 15 to 30 min in solution B, then 4 soft washes in Tris 0.05M with sodium metabisulfite 8.5g/l, pH 7.5 (solution C). 3- Tissue sectionning: Cryostat or vibratome sections can be used. 4- Reduction step: Sections are reduced with the solution C containing sodium borohydride (0.1M) for 10 min. Then, the sections are washed 4 times with solution C without sodium borohydride. 5- Application of anti-conjugated L.Tyrosine antibodies: The final dilution is 1/1,000 to 1/5,000 in solution C containing triton X100 0.5%, plus 2% of non-specific serum. A dozen of sections can be incubated with 2ml of antibody solution overnight at 4°C. Then, after this period, the sections are washed 3 times (10 min) with solution C. N.B.: Antibodies may be used at a higher dilution. The customer should explore the antibody dilution to reduce the possibility of high background. Note that a substitution in the buffer system as used in our protocol may change the background and the antibody recognition. 6- PAP procedure: Second antibody: Sections are incubated with 1/100 dilution of goat anti-rabbit in solution C for 3 hours at 20°C or 1 hour at 37°C. Then, they are washed 3 times (10 min) with solution C PAP: Sections are incubated with 1/1000 dilution of rabbit peroxidase anti-peroxidase complex in solution C for 1 hour at 37°C. Then, they are washed 3 times (10 min) with solution C Revelation: Antibody-antigen complexes are revealed using diaminobenzidine (25mg/100ml) (or other chromogen) dissolved in Tris 0.05M and filtrated 0.05% of H2O2 is added. The sections are incubated for 10 min at 20°C. Reaction is stopped by transfering sections in 5ml of Tris 0.05M. Gemacbio sells other antibodies to conjugated small molecules raised in rat or mouse: used together, these tools could be helpful for immunocytochemistry double labelling. Recommended dilutions for Western Blot (1/1,000-1/2,000) Recommended dilutions for Immunohistochemistry (1/1,000-1/5,000)
Purity	Antiserum previously preabsorbed on protein carriers, and purified
Storage	Lyophilized antibodies are stable at least 2 years.After reconstitution with 50µl of distilled water and 50µl of glycerol, the aliquot can be repeated freezed (up to five times).
Research Area	Amino Acids
Restrictions	For Research Use only

Publications: Tyrosine antibody

Publications

Kitahama, Geffard, Okamura et al.:
"Dopamine- and dopa-immunoreactive neurons in the cat forebrain with reference to tyrosine hydroxylase-immunohistochemistry."
In: Brain research , Vol. 518, Issue 1-2, pp. 83-94, 1990/01 (PubMed)

Trottier, Geffard, Evrard:
"Co-localization of tyrosine hydroxylase and GABA immunoreactivities in human cortical neurons."
In: Neuroscience letters , Vol. 106, Issue 1-2, pp. 76-82, 1990/01 (PubMed)

External Information: Tyrosine antibody

Google Scholar	Find more references for antigen Tyrosine on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Tyrosine (Bioinformatic Harvester (EMBL))