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Product details for Tyrosine antibody
Tyrosine antibody
Overview
| Antigen: | Tyrosine |
| Antibody Type: | Polyclonal |
| Host: | Rat |
| Quantity: | |
| Price: | 379,50 € (Plus shipping costs and VAT) |
| Order number: | ABIN107992 |
| Availability: | 7 days until shipment |
Additional Information: Tyrosine antibody
| Antigen | Tyrosine |
| Immunogen | Synthetic L.Tyrosine conjugated to protein carrier (Pc) |
| Antibody Type | Polyclonal |
| Format | Lyophilized |
| Isotype | IgG »Matching secondary antibodies |
| Host | Rat |
| Specificity | Conjugated L.Tyrosine . Using a conjugate L.Tyrosine-(Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Compounds Cross-reactivity ratio (a) L.Tyrosine-G-(Pc) 1 L-DOPA-G-(Pc) 1/400 Phenylalanine-G-(Pc) 1/1,000 Dopamine-G-(Pc) 1/>50,000 Noradrenaline-G-(Pc) 1/>50,000 (a): Tyrosine-G-(Pc) concentration / other conjugated catecholamine/amino acid concentration at half displacement G = Glutaraldehyde |
Application Details: Tyrosine antibody
| Application Notes | Example of Immunocytochemistry application Detection of conjugated L.Tyrosine in rat brain 1- Perfusion: The rat is anaesthetized with sodium Pentobarbital or Nembutal and perfused intracardially through the aorta using a pump with the following solutions: Solution A: cacodylate 0.1M, sodium metabisulfite 10g/l, pH = 6.2 Solution B: cacodylate 0.1M, sodium metabisulfite 10g/l and glutaraldehyde 3-5%, pH = 7.5 2- Post fixation: 15 to 30 min in solution B, then 4 soft washes in Tris 0.05M with sodium metabisulfite 8.5g/l, pH 7.5 (solution C). 3- Tissue sectionning: Cryostat or vibratome sections can be used. 4- Reduction step: Sections are reduced with the solution C containing sodium borohydride (0.1M) for 10 min. Then, the sections are washed 4 times with solution C without sodium borohydride. 5- Application of anti-conjugated L.Tyrosine antibodies: The final dilution is 1/1,000 to 1/5,000 in solution C containing triton X100 0.5%, plus 2% of non-specific serum. A dozen of sections can be incubated with 2ml of antibody solution overnight at 4°C. Then, after this period, the sections are washed 3 times (10 min) with solution C. N.B.: Antibodies may be used at a higher dilution. The customer should explore the antibody dilution to reduce the possibility of high background. Note that a substitution in the buffer system as used in our protocol may change the background and the antibody recognition. 6- PAP procedure: Second antibody: Sections are incubated with 1/100 dilution of goat anti-rat in solution C for 3 hours at 20°C or 1 hour at 37°C. Then, they are washed 3 times (10 min) with solution C PAP: Sections are incubated with 1/1000 dilution of rabbit peroxidase anti-peroxidase complex in solution C for 1 hour at 37°C. Then, they are washed 3 times (10 min) with solution C Revelation: Antibody-antigen complexes are revealed using diaminobenzidine (25mg/100ml) (or other chromogen) dissolved in Tris 0.05M and filtrated 0.05% of H2O2 is added. The sections are incubated for 10 min at 20°C. Reaction is stopped by transfering sections in 5ml of Tris 0.05M. Gemacbio sells other antibodies to conjugated small molecules raised in rat or mouse: used together, these tools could be helpful for immunocytochemistry double labelling. Recommended dilutions for Western Blot (1/1,000-1/2,000) Recommended dilutions for Immunochemistry (1/1,000-1/5,000) |
| Purity | Antiserum previously preabsorbed on protein carriers, and purified |
| Storage | Lyophilized antibodies are stable at least 2 years. After reconstitution with 50µl of distilled water and 50µl of glycerol, the aliquot can be repeated freezed (up to five times). |
| Research Area | Amino Acids |
| Restrictions | For Research Use only |
External Information: Tyrosine antibody
| Google Scholar | Find more references for antigen Tyrosine on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Tyrosine (Bioinformatic Harvester (EMBL)) |
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