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Product details for Urease Biotin (Jack Bean) antibody
Urease Biotin (Jack Bean) antibody
Overview
| Antigen: | Urease |
| Antibody Type: | Polyclonal |
| Application: | Enzyme Immunoassay (EIA), Western Blotting (WB) |
| Conjugate: | Biotin |
| Reactivity: | Jack Bean |
| Host: | Rabbit |
| Quantity: | |
| Price: | 340,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN116846 |
| Availability: | Ships in 3 to 10 days |
Additional Information: Urease Biotin (Jack Bean) antibody
| Antigen | Urease |
| Immunogen | Urease [Jack Bean] |
| Reactivity | Jack Bean |
| Antibody Type | Polyclonal |
| Host | Rabbit |
| Specificity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean]. Cross reactivities against Urease from other sources may occur but have not been specifically determined. |
Application Details: Urease Biotin (Jack Bean) antibody
| Application | Enzyme Immunoassay (EIA), Western Blotting (WB) |
| Application Notes | Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Urease in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. Other applications not tested. |
| Conjugate | Biotin |
| Concentration | 10.0 mg/ml (by UV absorbance at 280 nm) |
| Buffer | This antibody was lyophilised from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer. This antibody is conjugated to Biotinamidoca |
| Storage | Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. 1 |
| Research Area | Enzymes, Metabolism |
| Restrictions | Research only |
Publications: Urease Biotin (Jack Bean) antibody
| Publications |
Bayer, Wilchek: "Protein biotinylation." In: Methods in enzymology , Vol. 184, pp. 138-60, 1990/01 (PubMed) |
External Information: Urease Biotin (Jack Bean) antibody
| Google Scholar | Find more references for antigen Urease on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Urease (Bioinformatic Harvester (EMBL)) |
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