VEGFA antibody
Quick Overview for VEGFA antibody (ABIN116334)
Target
See all VEGFA AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Specificity
- Reacts with Vascular Endothelial Growth Factor (VEGF).
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Purification
- Affinity Chromatography.
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Immunogen
- E.coli derived highly pure (> 98%) recombinant Murine VEGF
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anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) antibody
VEGFA Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 28-147) antibodyVEGFA Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-214) antibodyVEGFA Reactivity: Rat WB, IHC, IP, ICC Host: Mouse Monoclonal C2 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) antibodyVEGFA Reactivity: Mouse WB, ELISA, Neut Host: Rabbit Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 207-371) antibodyVEGFA Reactivity: Human WB, ELISA, ICC Host: Mouse Monoclonal 6G5B5 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-191) antibodyVEGFA Reactivity: Human, Rat, Mouse WB, IHC, FACS Host: Rabbit Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 33-48), (N-Term) antibodyVEGFA Reactivity: Human, Rat, Mouse WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-130) antibodyVEGFA Reactivity: Human WB, ELISA, PLA Host: Mouse Monoclonal 3F7 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) antibodyVEGFA Reactivity: Human WB, ELISA, Neut Host: Goat Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) antibodyVEGFA Reactivity: Human WB, ELISA, Neut Host: Rabbit Polyclonal unconjugated
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Application Notes
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NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of mVEGF (50.00 ng/mL), a concentration of 1.0-2.0 μg/mL of this antibody is required. ELISAIndirect: To detect mVEGF by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well ofrecombinant mVEGF. Sandwich: To detect mVEGF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith biotinylated Anti-Murine VEGF (PP1113B) as a detection antibody, allows the detectionof at least 0.2-0.4 ng/well of recombinant mVEGF. Western BlotTo detect mVEGF by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Reconstitution
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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Buffer
- PBS, pH 7.2 without preservatives.
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Preservative
- Without preservative
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store the antibody at 2-8 °C for one month or at -20 °C for longer.
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- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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Alternative Name
- VEGF-A
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Background
- VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor
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Gene ID
- 22339
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NCBI Accession
- NP_001020421
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UniProt
- Q00731
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Pathways
- RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
Target
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