Product details for  alpha Tubulin antibody
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alpha Tubulin antibody

Overview

Image for alpha Tubulin antibody
Antigen: Alpha-Tubulin
Antibody Type: Monoclonal
Application: Western Blotting (WB), Immunocytochemistry (ICC)
Host: Mouse
Quantity: 1.0 mg (1 mg/ml) (Product variants)
Price: 480,00 €   (Plus shipping costs and VAT)
Order number: ABIN93890
Availability: 1 to 3 days until shipment
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Additional Information:  alpha Tubulin antibody

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Antigen Alpha-Tubulin
Immunogen Fraction of tubulin purified from pig brain by two cycles of polymerization-depolymerization.
Antibody Type Monoclonal
Format Purified
Isotype IgG1 »Matching secondary antibodies
Clone TU-01
Host Mouse
Specificity The antibody recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha tubulin.

Application Details:  alpha Tubulin antibody

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Application Western Blotting (WB), Immunocytochemistry (ICC)
Application Notes The Applications are presented only at purified format of antibody.
Concentration 1 mg/ml
Purity > 95% (by SDS-PAGE)
Purification Purified from ascites by precipitation methods.
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Storage Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
Restrictions For Research Use only

Publications:  alpha Tubulin antibody

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Publications Draber, Draberova, Linhartova et al.:
"Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies."
In: Journal of cell science , Vol. 92 ( Pt 3), pp. 519-28, 1990/01 (PubMed)

Draber, Draberova, Zicconi et al.:
"Heterogeneity of microtubules recognized by monoclonal antibodies to alpha-tubulin."
In: European journal of cell biology , Vol. 41, Issue 1, pp. 82-8, 1987/01 (PubMed)

Draber P, Draberova E, Viklicky V:
"Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head."
In: Histochemistry , Vol. 95, Issue 5, pp. 519-24, 1991/01 (PubMed)

Smertenko A, Blume Y, Viklicky V et al.:
"Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells."
In: Planta , Vol. 201, Issue 3, pp. 349-58, 1997/01 (PubMed)

Images:  alpha Tubulin antibody

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Fig. 1. Immunofluorescence staining of HeLa human cervix carcinoma epitheloid cell line with direct conjugate of alpha-tubulin specific monoclonal antibody TU-01/DY547 (red). Nucleus is stained with DAPI (blue).

Image for alpha Tubulin antibody (1)

Fig. 2. Immunofluorescence staining of 3T3 mouse embryonal fibroblasts with direct conjugate of alpha-tubulin specific monoclonal antibody TU-01/FITC (green). Nucleus is stained with DAPI (blue).

Image for alpha Tubulin antibody (2)

External Information:  alpha Tubulin antibody

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Google Scholar Find more references for clone TU-01 on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen alpha-Tubulin (Bioinformatic Harvester (EMBL))