alpha-DNA Topoisomerase I antibody

Overview

Antigen:	Alpha-DNA Topoisomerase I
Antibody Type:	Polyclonal
Application:	Western Blotting (WB)
Reactivity:	Human
Host:	Rabbit
Quantity:	50 µg
Price:	400,00 € (Plus shipping costs and VAT)
Order number:	ABIN123798
Availability:	Ships in 1 to 2 days.

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Additional Information: alpha-DNA Topoisomerase I antibody

Antigen	Alpha-DNA Topoisomerase I
Reactivity	Human
Antibody Type	Polyclonal
Format	Liquid. Supplied in PBS.
Description	Human DNA Topoisomerase I (Topo I) is a monomeric protein of 765 anino acids encoded by a single-copy gene. Based on its function, DNA Topoisomerase I has been subdivided into four distinct domains. The N-terminal 214 amino acids of Topo I comprise a highly charged N-terminal domain involved in protein-protein interactions with a number of cellular proteins. The highly conserved core domain expanded from amino acids 215 to 636 contains most catalytic residues and retains DNA binding activity. The active C-terminal domain (residues 713-765) is connected to the core domain by a poorly conserved linker domain (residues 636-712). Human DNA Topoisomerase I is the best studied of the DNA Topoisomerase family. It catalyzes the relaxation of both positive and negative supercoiled DNAs without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA Topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development. Since Topo I can cause large amounts of single-strand DNA breaks and this kind of DNA damage closely relates to most cancer, it is therefore believed that DNA Topo I can be an important target for antitumor agents. alpha-Topo I is an affinity-purified rabbit polyclonal antibody raised against a recombinant full-length DNA Topoisomerase I protein.
Host	Rabbit
Specificity	Alpha-Topo I reacts with the DNA Topoisomerase I protein in HeLa nuclear extract by Western blotting.

Application Details: alpha-DNA Topoisomerase I antibody

Application	Western Blotting (WB)
Application Notes	Recommended dilution range for Western blot analysis: 1:200 - 1:1000.
Storage	Store at -80 °C, avoid freeze/thaw cycles. Shipped on dry ice. Shelf Life: 12 months.
Restrictions	For Research Use only.

Publications: alpha-DNA Topoisomerase I antibody

Publications

Giovanella, Stehlin, Wall et al.:
"DNA topoisomerase I--targeted chemotherapy of human colon cancer in xenografts."
In: Science (New York, N.Y.) , Vol. 246, Issue 4933, pp. 1046-8, 1990/01 (PubMed)

Juan, Hwang, Liu et al.:
"Human DNA topoisomerase I is encoded by a single-copy gene that maps to chromosome region 20q12-13.2."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 85, Issue 23, pp. 8910-3, 1988/01 (PubMed)

Kretzschmar, Meisterernst, Roeder:
"Identification of human DNA topoisomerase I as a cofactor for activator-dependent transcription by RNA polymerase II."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 90, Issue 24, pp. 11508-12, 1994/01 (PubMed)

Liu, Miller:
"Eukaryotic DNA topoisomerases: two forms of type I DNA topoisomerases from HeLa cell nuclei."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 78, Issue 6, pp. 3487-91, 1981/01 (PubMed)

Pommier, Pourquier, Fan et al.:
"Mechanism of action of eukaryotic DNA topoisomerase I and drugs targeted to the enzyme."
In: Biochimica et biophysica acta , Vol. 1400, Issue 1-3, pp. 83-105, 1998/01 (PubMed)

Pourquier, Pilon, Kohlhagen et al.:
"Trapping of mammalian topoisomerase I and recombinations induced by damaged DNA containing nicks or gaps. Importance of DNA end phosphorylation and camptothecin effects."
In: The Journal of biological chemistry , Vol. 272, Issue 42, pp. 26441-7, 1997/01 (PubMed)

Redinbo, Stewart, Kuhn et al.:
"Crystal structures of human topoisomerase I in covalent and noncovalent complexes with DNA."
In: Science (New York, N.Y.) , Vol. 279, Issue 5356, pp. 1504-13, 1998/01 (PubMed)

Rossi, Labourier, Forne et al.:
"Specific phosphorylation of SR proteins by mammalian DNA topoisomerase I."
In: Nature , Vol. 381, Issue 6577, pp. 80-2, 1996/01 (PubMed)

Sekiguchi, Seeman, Shuman:
"Resolution of Holliday junctions by eukaryotic DNA topoisomerase I."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 93, Issue 2, pp. 785-9, 1996/01 (PubMed)

Stewart, Ireton, Parker et al.:
"Biochemical and biophysical analyses of recombinant forms of human topoisomerase I."
In: The Journal of biological chemistry , Vol. 271, Issue 13, pp. 7593-601, 1996/01 (PubMed)

Stewart, Ireton, Champoux:
"The domain organization of human topoisomerase I."
In: The Journal of biological chemistry , Vol. 271, Issue 13, pp. 7602-8, 1996/01 (PubMed)

DArpa, Machlin, Ratrie et al.:
"cDNA cloning of human DNA topoisomerase I: catalytic activity of a 67.7-kDa carboxyl-terminal fragment."
In: Proceedings of the National Academy of Sciences of the United States of America , Vol. 85, Issue 8, pp. 2543-7, 1988/01 (PubMed)

External Information: alpha-DNA Topoisomerase I antibody

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