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Product details for alphabeta TCR Rat antibody
alphabeta TCR Rat antibody
Overview
| Antigen: | Alphabeta TCR |
| Antibody Type: | Monoclonal |
| Application: | Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Activation, Depletion |
| Conjugate: | LEAF |
| Reactivity: | Rat, Cynomolgus |
| Host: | Mouse |
| Quantity: | |
| Price: | 347,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN157340 |
| Availability: | Ships within 7 to 10 days |
Additional Information: alphabeta TCR Rat antibody
| Antigen | Alphabeta TCR |
| Immunogen | Rat T cell blasts & erythrocytes |
| Reactivity | Rat, Cynomolgus |
| Antibody Type | Monoclonal |
| Format | Sterile Low-Endotoxin, Azide-Free formulation (LEAF) |
| Isotype | Mouse IgG1, kappa »Matching secondary antibodies |
| Description | TCR alpha and beta are members of the immunoglobulin superfamily and components of the CD3/TCR complex expressed on most peripheral T cells, intestinal epithelial lymphocytes and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation. The alpha/beta TCR recognizes peptide bound to MHC class I and II. The HR73 antibody has been shown to stimulate T cell activation in vitro. Distribution: Most peripheral T cells, intestinal epithelial lymphocytes, thymocytes Function: Antigen recognition, T cell activation/responses Synonyms: TCR alpha/beta, alpha/beta TCR Structure: Ig superfamily, CD3/TCR complex with CD3 and TCR-alpha subunit, 40 and 46 kD |
| Clone | R73 |
| Host | Mouse |
Application Details: alphabeta TCR Rat antibody
| Application | Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Activation, Depletion |
| Application Notes | Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, immunohistochemical staining of acetone-fixed frozen sections,in vitro activation of alpha/beta TCR T cells and in vivo depletion of alpha/beta TCR T cells . The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN157340). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Conjugate | LEAF |
| Purification | The antibody was purified by affinity chromatography. |
| Buffer | Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test. |
| Storage | The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions. |
| Research Area | Receptors |
| Restrictions | For Research Use only |
Publications: alphabeta TCR Rat antibody
| Publications |
Brenan, Rees: "Sequence analysis of rat integrin alpha E1 and alpha E2 subunits: tissue expression reveals phenotypic similarities between intraepithelial lymphocytes and dendritic cells in lymph." In: European journal of immunology , Vol. 27, Issue 11, pp. 3070-9, 1998/01 (PubMed) Fangmann, Schwinzer, Wonigeit: "Unusual phenotype of intestinal intraepithelial lymphocytes in the rat: predominance of T cell receptor alpha/beta+/CD2- cells and high expression of the RT6 alloantigen." In: European journal of immunology , Vol. 21, Issue 3, pp. 753-60, 1991/01 (PubMed) Huenig, Wallny, Hartley et al.: "A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes." In: The Journal of experimental medicine , Vol. 169, Issue 1, pp. 73-86, 1989/01 (PubMed) Mitnacht, Tacke, Huenig: "Expression of cell interaction molecules by immature rat thymocytes during passage through the CD4+8+ compartment: developmental regulation and induction by T cell receptor engagement of CD2, CD5, CD28, CD11a, CD44 and CD53." In: European journal of immunology , Vol. 25, Issue 2, pp. 328-32, 1995/01 (PubMed) Yoshino, Ami, Terao et al.: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." In: Experimental animals / Japanese Association for Laboratory Animal Science , Vol. 49, Issue 2, pp. 97-110, 2000/01 (PubMed) |
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