c-Fos Human antibody

Overview


	Antigen:	C-Fos
	Antibody Type:	Polyclonal
	Application:	Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
	Reactivity:	Human, Rat, Mouse (Murine)
	Host:	Rabbit
	Quantity:	500 µl (20 WB) (Product variants)
	Price:	289,00 € (Plus shipping costs and VAT)
	Order number:	ABIN161050
	Availability:	Ships within 7 to 10 days

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Additional Information: c-Fos Human antibody

Antigen	C-Fos
Immunogen	Peptide mapping to the amino terminus of human c-fos
Reactivity	Human, Rat, Mouse (Murine)
Antibody Type	Polyclonal
Format	Purified
Isotype	Rabbit IgG »Matching secondary antibodies
Description	The c-fos protein, also known as FBJ murine viral (v-fos) oncogene homolog , is a 62 kD cellular oncogene. The c-fos protein contains a bZIP region with a DNA-binding domain and a leucine zipper domain. Together with c-jun, c-jun proteins form the heterodimeric AP-1 complex that binds to the TRE elements of cellular and viral genes including collagenase, interleukin 2, stromelysin, SV40 and polyoma among others. c-fos is expressed in a variety of normal adult tissues and has been implicated as a critical transcription factor for skeletal formation and maintenance. This transcription factor is modified by phosphorylation by a number of upstream kinases and has been shown to interact with a variety of other proteins involved in transcriptional regulation. c-fos has been implicated in the development of fibrous bone dysplasia and osteosarcoma. The c-fos protein has been shown to interact with a variety of other proteins including c-jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1. The Poly6244 antibody recognizes human, rat, and mouse c-fos and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry. Distribution: Expressed in the nucleus of many tissues/cells including placenta, brain, thyroid, central nervous system, lung, endometrium, and B lymphocytes. Function: FOS and JUN oncoproteins form dimeric complexes that stimulate transcription of genes containing AP-1 regulatory elements. FOS was the first transcription factor identified to have a critical function in the development of cells required for skeletal formation and maintenance. Modifications: Phosphorylation on multiple sites (threonine, serine) by MAP kinases, ERK2, EphB2, PKA, Rsk. Synonyms: Oncogene Fos, FBJ murine viral (v-fos) oncogene homolog Regulation: c-fos levels are highly expressed in human term fetal tissues as compared to normal human tissues. c-fos levels have been reported to be increased in patients with fibrous bone dysplasia. Structure: c-fos heterodimerizes with c-jun to form the transcription factor AP-1, contains a bZIP region with a DNA-binding domain and a leucine zipper domain, approximately 62 kD.
Clone	Poly6244
Host	Rabbit
Specificity	Interacts with a variety of proteins including c-jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1.

Application Details: c-Fos Human antibody

Application	Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes	Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 25 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Purification	The antibody was purified by antigen-affinity chromatography.
Buffer	This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.
Storage	The antibody solution should be stored undiluted at 4°C. DO NOT FREEZE.
Restrictions	For Research Use only

Images: c-Fos Human antibody

Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit polyclonal antibody against c-fos. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.

Image for c-Fos Human antibody (1)

Product variants

c-Fos Human antibody (Format: Purified, Quantity: 125 µl (5 WB))

External Information: c-Fos Human antibody

Google Scholar	Find more references for clone Poly6244 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen c-Fos (Bioinformatic Harvester (EMBL))