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产品详细信息： C-erbB-2 (Dog (Canine)) antibody
C-erbB-2 (Dog (Canine)) antibody
详细信息: C-erbB-2 (Dog (Canine)) antibody
| 抗原 | |
| 免疫原 | Synthetic petide conjugated to KLH. |
| 目标物种 | Dog (Canine), Human, Mouse (Murine) |
| 抗体类型 | |
| 状态 | Purified |
| 抗体同种型 | IgG1 »相配的二抗 |
| 描述 | Molecular weight of antigen is 185kDa. |
| 抗体来源 | Mouse |
| 特异性 | ErbB2 is a member of the EGFR/erbB-receptor tyrosine kinase family. Dysregulation of erbB2 and/or activation of do wnstream signaling pathways has been implicated in many human cancers. ErbB2 is activated upon ligand dependent heterodimerization with EGFR, erbB3 or erbB4. ErbB2 homodimers are not favored due to the lack of an erbB2 specific extracellular ligand. Heterodimerization with EGFR or erbB4 leads to activation of the intrinsic tyrosine kinase activity of EGFR or erbB4 resulting in phosphorylation of multiple tyrosine residues within the erbB2 intracellular domain: Tyr 1023, Tyr 1112, Tyr 1139, Tyr 1196, Tyr 1222, and Tyr 1248. This antibody specifically recognizes the intracellular domain of erbB2. Epitope: intracellular domain (aa 860-880). Reacts with human. |
使用细节: C-erbB-2 (Dog (Canine)) antibody
| 应用范围 | Western Blotting (WB) |
| 应用备注 | Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% (v/v) Tween 20, 2x PBS. ELISA: 0.05 µg/ml. Positive control: SKOV-3, EGF-treated. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| 缓冲液 | This purified antibody lyophilized from 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 1 ml H2O (15 min, RT). |
| 贮藏 | For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freeze-thaw cycles. Shelf life: one year from despatch. |
| 研究领域 | Receptors, Growth Factors |
| 限制 | For Research Use only |
