PANIER
AIDE
Antigènes
Détail du produit pour C-erbB-2 (Dog (Canine)) antibody
C-erbB-2 (Dog (Canine)) antibody
Description du produit: C-erbB-2 (Dog (Canine)) antibody
| Antigène | |
| Immunogène | Synthetic petide conjugated to KLH. |
| Reactivité | Dog (Canine), Human, Mouse (Murine) |
| Type anticorps | |
| Format | Purified |
| Isotype | IgG1 »Anticorps secondaires correspondants |
| Description | Molecular weight of antigen is 185kDa. |
| Hôte | Mouse |
| Specificité | ErbB2 is a member of the EGFR/erbB-receptor tyrosine kinase family. Dysregulation of erbB2 and/or activation of do wnstream signaling pathways has been implicated in many human cancers. ErbB2 is activated upon ligand dependent heterodimerization with EGFR, erbB3 or erbB4. ErbB2 homodimers are not favored due to the lack of an erbB2 specific extracellular ligand. Heterodimerization with EGFR or erbB4 leads to activation of the intrinsic tyrosine kinase activity of EGFR or erbB4 resulting in phosphorylation of multiple tyrosine residues within the erbB2 intracellular domain: Tyr 1023, Tyr 1112, Tyr 1139, Tyr 1196, Tyr 1222, and Tyr 1248. This antibody specifically recognizes the intracellular domain of erbB2. Epitope: intracellular domain (aa 860-880). Reacts with human. |
Applications: C-erbB-2 (Dog (Canine)) antibody
| Application | Western Blotting (WB) |
| Indications d'application | Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% (v/v) Tween 20, 2x PBS. ELISA: 0.05 µg/ml. Positive control: SKOV-3, EGF-treated. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Buffer | This purified antibody lyophilized from 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 1 ml H2O (15 min, RT). |
| Stock | For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freeze-thaw cycles. Shelf life: one year from despatch. |
| Domaine de recherche | Receptors, Growth Factors |
| Restrictions | For Research Use only |
