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AIDE
Antigènes
Détail du produit pour C-erbB-2 (Dog (Canine)) antibody
C-erbB-2 (Dog (Canine)) antibody
Description du produit: C-erbB-2 (Dog (Canine)) antibody
| Antigène | |
| Immunogène | Synthetic phosphopetide conjugated to KLH. |
| Reactivité | Dog (Canine), Human, Mouse (Murine), Rat |
| Type anticorps | |
| Format | Purified |
| Isotype | IgG1 »Anticorps secondaires correspondants |
| Description | Molecular weight of antigen is 185kDa. |
| Hôte | Mouse |
| Specificité | ErbB2 is a member of the EGFR/erbB-receptor tyrosine kinase family. Dysregulation of erbB2 and/or activation of do wnstream signaling pathways has been implicated in many human cancers.ErbB2 is activated upon ligand dependent heterodimerizatio n with EGFR, erbB3 or erbB4. ErbB2 homodimers are not favored due to the lack of an erbB2 specific extracellular ligand. Heterodimerization with EGFR or erbB4 leads to activation of the intrinsic tyrosine kinase activity of EGFR or erbB4 resulting in phosphorylation of multiple tyrosine residues within the erbB2 intracellular domain: Tyr 1023, Tyr 1112, Tyr 1139, Tyr 1196, Tyr 1222, and Tyr 1248.Transphosphorylation via src family kinases leads to phosphorylation of Tyr 877, via PKC of Thr 669, via CamKinase2 of Ser 1113. Phosphorylation of Thr 669 and Ser 1113 interferes with erbB2 endocytosis and degradation This antibody specifically recognizes erbB2 phosphorylated at tyrosine 1112 at 185 kDa. Epitope: phospho-Tyr 1112. Reacts with human, mouse, rat and dog. |
Applications: C-erbB-2 (Dog (Canine)) antibody
| Application | Western Blotting (WB), Immunofluorescence (IF) |
| Indications d'application | Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% (v/v) Tween 20, 2x PBS. ELISA: 0.05 µg/ml. Positive control: SKOV-3, EGF-treated. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Buffer | This purified antibody lyophilized from 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 1 ml H2O (15 min, RT). |
| Stock | Aliquote and freeze in liquid nitrogen, reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freeze-thaw cycles. Shelf life: one year from despatch. |
| Domaine de recherche | Receptors, Growth Factors, Phospho-specific antibodies |
| Restrictions | For Research Use only |
