c-erbB-2 (Human) antibody

Overview

Antigen:	C-erbB-2 / Her-2 / neu
Antibody Type:	Polyclonal
Application:	Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Reactivity:	Mouse (Murine), Human
Host:	Rabbit
Quantity:	50 (0.2 mg/ml)
Price:	200,00 € (Plus shipping costs and VAT)
Order number:	ABIN123230
Availability:	Ships in 3 to 10 days

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Additional Information: c-erbB-2 (Human) antibody

Antigen	C-erbB-2 / Her-2 / neu
Immunogen	Synthetic peptide from C-terminus of human c-erbB-2 protein
Reactivity	Mouse (Murine), Human
Antibody Type	Polyclonal
Format	Aff - Purified
Host	Rabbit
Specificity	The c-erbB-2 (HER-2/neu) proto-oncogene encodes a 185 kDa transmembrane receptor protein. It has partial homology with the epidermal growth factor receptor and shares intrinsic tyrosine kinase activity with that receptor. c-erbB-2 can heterodimerize with the EGF receptor, erbB-3 receptor, and the erbB-4 receptor. HER-2/neu was originally identified as a transforming oncogene in chemically induced rat neuroglioblastomas, where a single point mutation in the transmembrane domain of the molecule is sufficient to confer oncogenic activation. In contrast, in human breast and ovarian cancers the receptor is activated through amplification and overexpression of the wild-type gene. This alteration occurs in 25%-30% of cases and is associated with a poor prognosis. The poor prognosis of ovarian and breast cancer patients associated with c-erbB-2 overexpression seemed to be sufficiently explained by a c-erbB-2-mediated high rate of tumor cell proliferation. Amplification of c-erbB-2 proto-oncogene has been reported in endocrine tumors. c-erbB-2 may play a role in the genesis of human gastric, endometrial and salivary gland cancers. The concentration of c-erbB-2 protein in serum can be a sensitive indicator for prognosis of the gastric carcinoma that express c-erbB-2. The c-erbB-2 protein expression might be enhanced in advanced stages during the progression of gastric carcinoma. c-erbB-2 oncoprotein is detectable in adenocarcinomas as well as in transitional cell carcinomas. Topoisomerase II, which correlates with c-erbB-2 expression, contributes to the resistance of c-erbB-2 overexpressing carcinomas. This antibody reacts specifically with human c-erbB-2 (HER-2/neu).

Application Details: c-erbB-2 (Human) antibody

Application	Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Application Notes	ELISA (0.1 – 1.0 µg/ml). Western blot (0.5 – 2.0 µg/ml). Immunoprecipitation (3.0 – 5.0 µg / extract from 107 cells). Immunohistochemistry frozen and formalin-fixed, paraffin embedded tissue sections (1/300, Staining of paraffin embedded tissues requires boilin g of tissue sections in 10 mM citrate buffer pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes, The staining pattern is cell membrane. Positive control is breast carcinoma or SKBR3 cells). Other applications not tested. Optimal dilutions depend on working conditions (e.g. secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature and length of incubations) and should be determined by the user.
Concentration	0.2 mg/ml
Buffer	The antibody is supplied as epitope affinity purified rabbit IgG fraction, in Phosphate-buffered saline pH 7.4, containing BSA as stabilizer and 0.09% Sodium Azide as preservative.
Storage	Store at 4°C for up to one month. Store at –20°C for long term storage. Avoid repeated freezing and tha wing. Shelf life: one year from despatch.
Research Area	Receptors, Growth Factors
Restrictions	Research only

Publications: c-erbB-2 (Human) antibody

Publications

Lozupone, Favia, Cafagna et al.:
"[Quantitative variations of osteocalcin in the culture media of bones subjected to intermittent mechanical loads in vitro]"
In: Bollettino della Società italiana di biologia sperimentale , Vol. 69, Issue 4, pp. 209-14, 1994/01 (PubMed)

Ruiz-Ruiz, Samperiz Legarre, Ruiz Laiglesia et al.:
"[Influence of age in mortality of patients with heart failure]"
In: Anales de medicina interna (Madrid, Spain : 1984) , Vol. 22, Issue 9, pp. 424-8, 2006/01 (PubMed)

External Information: c-erbB-2 (Human) antibody

Google Scholar	Find more references for antigen c-erbB-2 / Her-2 / neu on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen c-erbB-2 / Her-2 / neu (Bioinformatic Harvester (EMBL))