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产品详细信息： c-fos pSer374 (Dog (Canine)) antibody
c-fos pSer374 (Dog (Canine)) antibody
概述
| 抗原: | C-fos pSer374 () |
| 抗体类型: | 单克隆抗体 |
| 应用范围: | Enzyme Immunoassay (EIA), Western Blotting (WB) |
| 目标物种: | Dog (Canine), Human |
| 抗体来源: | Mouse |
| 规格: | |
| 价格: | 330,00 € (加上运费和增值税) |
| 货号: | ABIN126800 |
| 发货时间: | 3 to 10 days until shipment |
详细信息: c-fos pSer374 (Dog (Canine)) antibody
| 抗原 | C-fos pSer374 () |
| 免疫原 | Synthetic phosphopeptide conjugated to KLH. |
| 目标物种 | Dog (Canine), Human |
| 抗体类型 | 单克隆抗体 |
| 状态 | Purified |
| 抗体同种型 | IgG1 »相配的二抗 |
| 描述 | Molecular weight of antigen is 50kDa. |
| 克隆 | 3-40E-005 |
| 抗体来源 | Mouse |
| 特异性 | The immediated early gene product c-Fos is expressed following mitogenic stimulation. c-Fos functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at serine 374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at threonine 325. This antibody specifically interacts with c-Fos phosphorylated at serine 374 and does not crossreact with the non-phosphorylated c-Fos nor with unrelated serine-phosphorylated proteins. The antibody is suitable for Western blot and ELISA applications. Epitope: S L S pS P T L (phosphoserine 374). Reacts with human and dog. |
使用细节: c-fos pSer374 (Dog (Canine)) antibody
| 应用范围 | Enzyme Immunoassay (EIA), Western Blotting (WB) |
| 应用备注 | Western Blot: 0.5 µg/ml for HRPO/ECL detection Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% (v/v) Tween 20, 2x PBS. ELISA: 0.1 µg/ml (protein ELISA), capture ELISA: not determined. Positive control: HepG2 EGF-treated. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| 缓冲液 | This purified antibody lyophilized from 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 1 ml H2O (15 min, RT). |
| 贮藏 | For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freeze-thaw cycles. Shelf life: one year from despatch. |
| 限制 | For Research Use only |
外部连接: c-fos pSer374 (Dog (Canine)) antibody
| Google Scholar | 克隆 3-40E-005 |
| EMBL Harvester | 在human, mouse 或 rat 基因组里搜索抗原c-fos pSer374 (incl. pos control) (Bioinformatic Harvester (EMBL)) |
