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Détail du produit pour c-fos pSer374 (Dog (Canine)) antibody
c-fos pSer374 (Dog (Canine)) antibody
Aperçu
| Antigène: | C-fos pSer374 () |
| Type anticorps: | Monoclonal |
| Application: | Enzyme Immunoassay (EIA), Western Blotting (WB) |
| Reactivité: | Dog (Canine), Human |
| Hôte: | Mouse |
| Quantité: | |
| Prix: | 330,00 € (Plus frais de livraison et TVA) |
| Numéro de commande: | ABIN126800 |
| Disponibilité: | 3 to 10 days until shipment |
Description du produit: c-fos pSer374 (Dog (Canine)) antibody
| Antigène | C-fos pSer374 () |
| Immunogène | Synthetic phosphopeptide conjugated to KLH. |
| Reactivité | Dog (Canine), Human |
| Type anticorps | Monoclonal |
| Format | Purified |
| Isotype | IgG1 »Anticorps secondaires correspondants |
| Description | Molecular weight of antigen is 50kDa. |
| Clone | 3-40E-005 |
| Hôte | Mouse |
| Specificité | The immediated early gene product c-Fos is expressed following mitogenic stimulation. c-Fos functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at serine 374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at threonine 325. This antibody specifically interacts with c-Fos phosphorylated at serine 374 and does not crossreact with the non-phosphorylated c-Fos nor with unrelated serine-phosphorylated proteins. The antibody is suitable for Western blot and ELISA applications. Epitope: S L S pS P T L (phosphoserine 374). Reacts with human and dog. |
Applications: c-fos pSer374 (Dog (Canine)) antibody
| Application | Enzyme Immunoassay (EIA), Western Blotting (WB) |
| Indications d'application | Western Blot: 0.5 µg/ml for HRPO/ECL detection Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% (v/v) Tween 20, 2x PBS. ELISA: 0.1 µg/ml (protein ELISA), capture ELISA: not determined. Positive control: HepG2 EGF-treated. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user. |
| Buffer | This purified antibody lyophilized from 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 1 ml H2O (15 min, RT). |
| Stock | For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freeze-thaw cycles. Shelf life: one year from despatch. |
| Restrictions | For Research Use only |
Informations supplémentaires: c-fos pSer374 (Dog (Canine)) antibody
| Google Scholar | Trouver des références supplémentaires pour clone 3-40E-005 sur Google Scholar™ |
| EMBL Harvester | Recherche humain, souris ou rat génome pour antigène c-fos pSer374 (incl. pos control) (Bioinformatic Harvester (EMBL)) |
