Product details for  p55 subunit of human Transcription Factor II A antibody
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p55 subunit of human Transcription Factor II A antibody

Overview

Antigen: P55 subunit of human Transcription Factor II A
Antibody Type: Polyclonal
Application: Western Blotting (WB)
Reactivity: Human
Host: Rabbit
Quantity: 50 µg
Price: 400,00 €   (Plus shipping costs and VAT)
Order number: ABIN123787
Availability: Ships in 1 to 2 days.
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Additional Information:  p55 subunit of human Transcription Factor II A antibody

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Antigen P55 subunit of human Transcription Factor II A
Reactivity Human
Antibody Type Polyclonal
Format Liquid. Supplied in PBS.
Description The transcription factor IIA (TFIIA) has been shown to bind to the TBP-DNA complex and to increase the affinity of TBP for the TATA element. Human TFIIA consists of three subunits of 35 kDa (alpha-subunit), 19 kDa (beta-subunit) and 12 kDa (gamma-subunit). The alpha- and beta-subunits are derived from the product, p55, of a single gene by an unknown mechanism. However, recombinant p55, in combination with a 12 kDa subunit (gamma-subunit), retains native TFIIA activity. alpha-TFIIA is an affinity-purified rabbit polyclonal antibody raised against a recombinant full-length p55 subunit of the TFIIA complex.
Host Rabbit
Specificity Alpha-TFIIA reacts with the p55 protein in HeLa nuclear extract by Western blotting.

Application Details:  p55 subunit of human Transcription Factor II A antibody

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Application Western Blotting (WB)
Application Notes Recommended dilution range for Western blot analysis: 1:200 - 1:1000.
Storage Store at -80 °C, avoid freeze/thaw cycles. Shipped on dry ice. Shelf Life: 12 months.
Restrictions For Research Use only.

Publications:  p55 subunit of human Transcription Factor II A antibody

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Publications Buratowski, Hahn, Guarente et al.:
"Five intermediate complexes in transcription initiation by RNA polymerase II."
In: Cell , Vol. 56, Issue 4, pp. 549-61, 1989/01 (PubMed)

DeJong, Roeder:
"A single cDNA, hTFIIA/alpha, encodes both the p35 and p19 subunits of human TFIIA."
In: Genes & development , Vol. 7, Issue 11, pp. 2220-34, 1993/01 (PubMed)

Ranish, Hahn:
"The yeast general transcription factor TFIIA is composed of two polypeptide subunits."
In: The Journal of biological chemistry , Vol. 266, Issue 29, pp. 19320-7, 1991/01 (PubMed)

External Information:  p55 subunit of human Transcription Factor II A antibody

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Google Scholar Find more references for antigen alpha-hTFIIA (anti-p55) on Google Scholar™
EMBL Harvester Search human, mouse or rat genome for antigen alpha-hTFIIA (anti-p55) (Bioinformatic Harvester (EMBL))