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Product details for Histone H3 - Methyl K9 (C. elegans) antibody
Histone H3 - Methyl K9 (C. elegans) antibody
Overview
| Antigen: | Histone H3 - Methyl K9 |
| Antibody Type: | Polyclonal |
| Application: | Western Blotting (WB) |
| Reactivity: | Human, Mouse (Murine), Rat, Drosophila, Caenorhabditis Elegens |
| Host: | Rabbit |
| Quantity: | |
| Price: | 330,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN151631 |
| Availability: | Ships in 7 to 10 days |
Additional Information: Histone H3 - Methyl K9 (C. elegans) antibody
| Antigen | Histone H3 - Methyl K9 |
| Immunogen | Synthetic peptide corresponding to amino acids 7-20 of Histone H3 conjugated to KLH. |
| Reactivity | Human, Mouse (Murine), Rat, Drosophila, Caenorhabditis Elegens |
| Antibody Type | Polyclonal |
| Format | Aff - Purified |
| Description | The relatively unstructured and highly charged N-terminal tail domains of histones arecentral to the processes that modulate chromatin structure. A diverse and elaboratearray of post-translational modifications, including acetylation, phosphorylation, andmethylation occurs on the N-terminal tail domains of histones, particularly of H3 andH4.1, 2 These modifications may alter chromatin structure and recruit downstreamchromatin-associated proteins involved in transcription regulation. These in turn maydictate dynamic transitions between transcriptionally active or silent chromatin states.Histones H3 and H4 are the predominant histones modified by methylation and arehighly methylated in mammalian cells.3, 4 Histone methylation, like acetylation, is acomplex, dynamic process involving a number of processes, including transcriptionalregulation, chromatin condensation, mitosis, and heterochromatin assembly. Moreover,lysine residues can be mono-, di-, and tri-methylated, adding further complexity to theregulation of chromatin structure.Conserved lysine residues in the N-terminal tail domains of histone H3, Lys4, Lys9 andLys27 are the preferred sites of methylation.1, 4-6 Methylation of H3 at Lys9 is amodification intrinsically linked to epigenetic silencing and heterochromatin assembly.Histone H3 is methylated at Lys9 by site-specific H3 methyltransferases (HMTases)encoded by the SUV39H1 gene family.7 Methylation of H3 at Lys9 by SUV39H1generates a binding site for HP1 proteins, a family of heterochromatic adaptor proteinsimplicated in both gene silencing and in the organization of higher order chromatin.8-11Methylation of Lys9 interferes with the phosphorylation of Ser10 but is also influencedby preexisting modifications in the N-terminus of H3, such as H3 Ser10 phosphorylationitself.7 Conversely, in vivo deregulated SUV39H1 or disrupted SUV39H1activitymodulates H3 Ser10 phosphorylation in native chromatin leading to aberrant mitoticdivisions. Synonyms: H3, HisH3, His1H3, H3/a, H3/b, H3,c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, HistoneH3 |
| Host | Rabbit |
| Specificity | Detects a band at approximately 17kDa. This Histone H3 sequences is identical in manyspecies including mouse, rat, bovine, chicken, frog, drosophila and C. elegans. There isno inhibition with the non-methylated Histone H3 peptide. Cross-reacts with Human. Expected to cross react with mouse, rat, bovine, chicken,frog, drosophila and C. elegans. |
Application Details: Histone H3 - Methyl K9 (C. elegans) antibody
| Application | Western Blotting (WB) |
| Application Notes | Suggested working dilutions *Western Blot * Investigator should determine optimal working dilutions. WB: Use at a minimum dilution of 1/1,000. Not tested in any other applications. Optimaldilution should be determined by the end user. * Other applications have not been tested. Positive Control:epitheloid carcinoma HeLa cells |
| Concentration | 0.1 mg/ml |
| Buffer | 0.01 PBS, pH 7.4 containing 1.0% BSA and 15mM sodium azide 50 microliters |
| Storage | Store at 4 degress Celcius for up to one month. Aliquot and store at -20 degrees Celciuslong term. Avoid repeated freeze/thaw cycles. |
| Research Area | DNA/RNA, Transcription Factors |
| Restrictions | For Research Use only |
External Information: Histone H3 - Methyl K9 (C. elegans) antibody
| Google Scholar | Find more references for antigen Histone H3 - Methyl K9 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen Histone H3 - Methyl K9 (Bioinformatic Harvester (EMBL)) |
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