anti-Mouse (Murine) AIFM1 antibody for Western Blotting

Recommended AIFM1 Antibody (supplied by: Log in to see )

Antigen
Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) Antibodies
  • aif
  • pdcd8
  • PDCD8
  • AIFM1
  • AIF
  • PCD8
  • CG7263
  • DmAIF
  • Dmel\\CG7263
  • GB16024
  • DDBDRAFT_0187853
  • DDBDRAFT_0191137
  • DDB_0187853
  • DDB_0191137
  • AIFsh2
  • Hq
  • Pdcd8
  • mAIF
  • Aif
  • zgc:91994
  • CMTX4
  • COWCK
  • COXPD6
  • apoptosis-inducing factor, mitochondrion-associated, 1
  • Apoptosis inducing factor
  • apoptosis inducing factor
  • allograft inflammatory factor 1
  • apoptosis-inducing factor, mitochondrion-associated 1
  • aifm1
  • AIFM1
  • AIF
  • aif
  • AIF1
  • Aifm1
Epitope
Middle Region
28
15
14
13
10
5
4
4
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
178
77
74
19
10
9
6
4
2
2
2
1
1
1
1
1
1
1
Host
Rabbit
139
32
6
2
Clonality
Polyclonal
Conjugate
This AIFM1 antibody is un-conjugated
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
156
62
51
35
26
19
13
11
10
6
5
3
3
2
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Catalog No. ABIN2792122
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1524868 ABIN222988 WB Rabbit IgG Log in to see Polyclonal 2
3.1524868 ABIN1534424 ELISA WB Rabbit IgG Log in to see Polyclonal 2
3.1524868 ABIN3043779 ICC IHC (p) WB Rabbit IgG AA 582-613, C-Term Log in to see Polyclonal
3.1524868 ABIN724010 IF (p) IHC (p) WB Rabbit IgG AA 130-180 Log in to see Polyclonal 1
3.1524868 ABIN968950 ICC FACS IHC ELISA WB Mouse IgG2b Log in to see 4-00E-07 1
3.1524868 ABIN4278849 ELISA WB Goat C-Term, Isoform 1 Log in to see Polyclonal 1
3.1524868 ABIN2477307 WB Rabbit IgG AA 593-606 Log in to see Polyclonal 1
3.1524868 ABIN2778665 IHC WB Rabbit C-Term Log in to see Polyclonal
3.1524868 ABIN3044471 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 596-613, C-Term Log in to see Polyclonal
3.1524868 ABIN967344 WB Rabbit Ig Fraction Log in to see Polyclonal 3
3.1524868 ABIN1105269 EIA IF IHC (p) WB Mouse IgG2b Log in to see 4-00E-007 1
3.1524868 ABIN4278824 ELISA IHC IHC (p) WB Rabbit Center, all Isoforms Log in to see Polyclonal
3.1524868 ABIN2707315 IC IF IP IHC WB Rabbit N-Term Log in to see Polyclonal
3.1524868 ABIN1870886 IF IHC IP WB Rabbit IgG Log in to see Polyclonal
3.1524868 ABIN2462044 ELISA WB Rabbit Log in to see Polyclonal
3.1524868 ABIN261023 IHC (p) IHC WB Rabbit Log in to see Polyclonal
3.1524868 ABIN4278818 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
3.1524868 ABIN4278820 ELISA FACS ICC IF IHC WB Mouse IgG2b Log in to see 4E7
3.1524868 ABIN4278826 IHC (fro) IHC (p) IP SimWes WB Rabbit AA 151-170 Log in to see Polyclonal
3.1524868 ABIN408863 IHC (p) IHC WB Rabbit Log in to see Polyclonal

Top referenced anti-AIFM1 antibody for Western Blotting

  • Human Polyclonal AIFM1 Primary Antibody for WB - ABIN222988 (2 Publications): Jayanthi, Deng, Noailles, Ladenheim, Cadet: Methamphetamine induces neuronal apoptosis via cross-talks between endoplasmic reticulum and mitochondria-dependent death cascades. in FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2004 (PubMed)

  • Human Polyclonal AIFM1 Primary Antibody for WB - ABIN967344 (3 Publications): Joza, Susin, Daugas, Stanford, Cho, Li, Sasaki, Elia, Cheng, Ravagnan, Ferri, Zamzami, Wakeham, Hakem, Yoshida, Kong, Mak, Zúñiga-Pflücker, Kroemer, Penninger: Essential role of the mitochondrial apoptosis-inducing factor in programmed cell death. in Nature 2001 (PubMed)

  • Human Polyclonal AIFM1 Primary Antibody for ELISA, WB - ABIN4278849 (1 Publications): Lewis, Wilkinson, Jackson, Mehra, Varambally, Chinnaiyan, Wilkinson: The enzymatic activity of apoptosis-inducing factor supports energy metabolism benefiting the growth and invasiveness of advanced prostate cancer cells. in The Journal of biological chemistry 2012 (PubMed)

  • Human Polyclonal AIFM1 Primary Antibody for IF (p), IHC (p) - ABIN724010 (1 Publications): Gong, Hua, Zhang, Zhao, Tang, Mei, Zhang, Cui, Li: Hypothermia-induced neuroprotection is associated with reduced mitochondrial membrane permeability in a swine model of cardiac arrest. in Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2013 (PubMed)

  • Human Monoclonal AIFM1 Primary Antibody for ICC, FACS - ABIN968950 (1 Publications): Son, Jang, Heo, Chung, Choi, Lee: Apoptosis-inducing factor plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. in Apoptosis : an international journal on programmed cell death 2009 (PubMed)

Similar anti-AIFM1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Dog (Canine) Rabbit Pig (Porcine) Horse (Equine)
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies 3 Antibodies 3 Antibodies
Simple Western (SimWes) 2 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 156 Antibodies 64 Antibodies 61 Antibodies 10 Antibodies 11 Antibodies 3 Antibodies 8 Antibodies 3 Antibodies
Immunoprecipitation (IP) 10 Antibodies 2 Antibodies 2 Antibodies 8 Antibodies

General

Antigen Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) Antibodies
Epitope Middle Region
(28), (15), (14), (13), (10), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(178), (77), (74), (19), (10), (9), (6), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(139), (32), (6), (2)
Clonality
Polyclonal
Conjugate This AIFM1 antibody is un-conjugated
(4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(156), (62), (51), (35), (26), (19), (13), (11), (10), (6), (5), (3), (3), (2)
Pubmed 1 reference available
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Product Details anti-AIFM1 Antibody

Target Details AIFM1 Application Details Handling ProductDetails: References for anti-AIFM1 antibody (ABIN2792122) Images
Sequence VIFYLRDKVV VGIVLWNIFN RMPIARKIIK DGEQHEDLNE VAKLFNIHED
Predicted Reactivity Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against AIFM1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human AIFM1

Target Details AIFM1

Product Details anti-AIFM1 Antibody Application Details Handling ProductDetails: References for anti-AIFM1 antibody (ABIN2792122) Images back to top
Antigen
Alternative Name AIFM1 (AIFM1 Antibody Abstract)
Background AIFM1 is a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this protein induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Several alternative transcripts encoding different isoforms have been identified for this gene.This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Three alternative transcripts encoding different isoforms have been identified for this gene.
Alias Symbols: AIF, MGC111425, PDCD8, COXPD6
Protein Interaction Partner: ISG15, STAU1, BAG6, UBC, NEDD8, MDM2, ASB12, RNF2, BMI1, SUZ12, PARK2, BAG3, ADRB2, HDAC11, OXSR1, ILK, PAN2, NOS2, MLH1, CFTR, ESR1, TST, FSCN1, CPOX, SHC1, FBXO6, TSC22D4, CAND1, COPS5, CUL1, CUL2, CUL3, CUL5, TONSL, BLNK, PIDD1, XIAP, AMFR, Tubb4b, Tub
Protein Size: 613
Molecular Weight 67 kDa
Gene ID 9131
NCBI Accession NM_004208, NP_004199
UniProt O95831
Pathways Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration

Application Details

Product Details anti-AIFM1 Antibody Target Details AIFM1 Handling ProductDetails: References for anti-AIFM1 antibody (ABIN2792122) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 613 AA

Restrictions For Research Use only

Handling

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details ProductDetails: References for anti-AIFM1 antibody (ABIN2792122) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-AIFM1 antibody (ABIN2792122)

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details Handling Images back to top
Background publications

Desmots, Russell, Michel, McKinnon: "Scythe regulates apoptosis-inducing factor stability during endoplasmic reticulum stress-induced apoptosis." in: The Journal of biological chemistry, Vol. 283, Issue 6, pp. 3264-71, 2008

Images

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details Handling ProductDetails: References for anti-AIFM1 antibody (ABIN2792122) back to top
Supplier Images
Western Blotting (WB) image for anti-AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1) (Middle Region) (ABIN2792122) WB Suggested Anti-AIFM1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Western Blotting (WB) image for anti-AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1) (Middle Region) (ABIN2792122) WB Suggested Anti-AIFM1 Antibody
Titration: 1 ug/ml
Positive ...
Western Blotting (WB) image for anti-AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1) (Middle Region) (ABIN2792122) WB Suggested Anti-AIFM1
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:1562500...