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anti-Rat (Rattus) AIFM1 antibody for Enzyme Immunoassay

Recommended AIFM1 Antibody (supplied by: Log in to see )

Antigen
Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) Antibodies
  • aif
  • Aif
  • AIF
  • AIFM1
  • AIFsh2
  • CG7263
  • CMTX4
  • COWCK
  • COXPD6
  • DDBDRAFT_0187853
  • DDBDRAFT_0191137
  • DDB_0187853
  • DDB_0191137
  • DmAIF
  • Dmel\\CG7263
  • GB16024
  • Hq
  • mAIF
  • PCD8
  • PDCD8
  • pdcd8
  • Pdcd8
  • zgc:91994
Epitope
AA 593-606, C-Term
38
16
16
15
13
5
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
174
67
63
15
9
8
5
5
4
3
2
1
1
1
1
1
1
Host
Rabbit
160
17
2
2
Clonality
Polyclonal
Conjugate
This AIFM1 antibody is un-conjugated
4
4
4
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
155
74
46
36
26
18
11
10
8
5
4
3
2
Supplier
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Catalog No. ABIN499239
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008899843 ABIN499237 EIA IHC (p) WB Rabbit IgG Intermediate Domain Log in to see Polyclonal 3
0.0008899843 ABIN1105269 EIA IF IHC (p) WB Mouse IgG2b Log in to see 4-00E-007 1

Top referenced anti-AIFM1 antibody for Enzyme Immunoassay

Similar anti-AIFM1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 23 Antibodies 26 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 3 Antibodies
Flow Cytometry (FACS) 2 Antibodies 2 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 9 Antibodies 9 Antibodies
Immunofluorescence (IF) 7 Antibodies 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 21 Antibodies 23 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 54 Antibodies

General

Antigen Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) Antibodies
Epitope AA 593-606, C-Term
(38), (16), (16), (15), (13), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(174), (67), (63), (15), (9), (8), (5), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(160), (17), (2), (2)
Clonality
Polyclonal
Conjugate This AIFM1 antibody is un-conjugated
(4), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(155), (74), (46), (36), (26), (18), (11), (10), (8), (5), (4), (3), (2)
Pubmed 2 references available
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Product Details anti-AIFM1 Antibody

Target Details AIFM1 Application Details Handling References for anti-AIFM1 Antibody (ABIN499239) Images
Specificity This antibody detects AIF at C-term.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse, Rat
Purification Affinity chromatography purified via peptide column
Immunogen Peptide corresponding to amino acids 593 to 606 of Human AIF .Remarks: This sequence is identical to those of Mouse and Rat AIF.
Isotype IgG

Target Details AIFM1

Product Details anti-AIFM1 Antibody Application Details Handling References for anti-AIFM1 Antibody (ABIN499239) Images back to top
Antigen
Alternative Name AIFM1 / AIF (AIFM1 Antibody Abstract)
Background Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed (2).Synonyms: Apoptosis-inducing factor 1 mitochondrial, PDCD8, Programmed cell death protein 8
Gene ID 9131
NCBI Accession NP_004199
UniProt O95831
Pathways Apoptosis

Application Details

Product Details anti-AIFM1 Antibody Target Details AIFM1 Handling References for anti-AIFM1 Antibody (ABIN499239) Images back to top
Application Notes ELISA. Western blot: 0.25 to 1 μg/mL. A 67 kDa band should be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details References for anti-AIFM1 Antibody (ABIN499239) Images back to top
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-AIFM1 Antibody (ABIN499239)

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details Handling Images back to top
Background publications

Zamzami, Kroemer: "Condensed matter in cell death." in: Nature, Vol. 401, Issue 6749, pp. 127-8, 1999

Susin, Lorenzo, Zamzami, Marzo, Snow, Brothers, Mangion, Jacotot, Costantini, Loeffler, Larochette, Goodlett, Aebersold, Siderovski, Penninger, Kroemer: "Molecular characterization of mitochondrial apoptosis-inducing factor." in: Nature, Vol. 397, Issue 6718, pp. 441-6, 1999

Images

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details Handling References for anti-AIFM1 Antibody (ABIN499239) back to top
Supplier Images
Immunofluorescence (IF) image for anti-AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1) (AA 593-606) (ABIN499239) Immunocytochemistry of AIF in K562 cells with AP30031PU-N AIF antibody at 5 μg/ml.
Western Blotting (WB) image for anti-AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1) (AA 593-606) (ABIN499239) Western blot analysis of AIF in K562 with AP30031PU-N AIF antibody at (A) 0.5, (B) 1,...