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anti-Rat (Rattus) AIFM1 antibody for Immunohistochemistry (Frozen Sections)

Recommended AIFM1 Antibody (supplied by: Log in to see )

Antigen
Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) Antibodies
  • aif
  • Aif
  • AIF
  • AIFM1
  • AIFsh2
  • CG7263
  • CMTX4
  • COWCK
  • COXPD6
  • DDBDRAFT_0187853
  • DDBDRAFT_0191137
  • DDB_0187853
  • DDB_0191137
  • DmAIF
  • Dmel\\CG7263
  • GB16024
  • Hq
  • mAIF
  • PCD8
  • PDCD8
  • pdcd8
  • Pdcd8
  • zgc:91994
Epitope
AA 596-613, C-Term
41
19
17
15
13
5
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
190
75
71
16
11
10
5
5
4
3
2
1
1
1
1
1
1
1
Host
Rabbit
172
19
4
2
Clonality
Polyclonal
Conjugate
This AIFM1 antibody is un-conjugated
4
4
4
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
171
82
51
42
30
21
13
11
9
6
4
4
2
2
Supplier
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Catalog No. ABIN3044471
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000883884 ABIN4278826 IHC (fro) IHC (p) IP SimWes WB Rabbit AA 151-170 Log in to see Polyclonal
0.000883884 ABIN4278825 IHC (fro) IHC (p) IP SimWes WB Rabbit AA 151-170 Log in to see Polyclonal

Similar anti-AIFM1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 28 Antibodies 31 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 3 Antibodies
Flow Cytometry (FACS) 3 Antibodies 3 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 11 Antibodies 11 Antibodies
Immunofluorescence (IF) 9 Antibodies 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 24 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Western Blotting (WB) 62 Antibodies

General

Antigen Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) Antibodies
Epitope AA 596-613, C-Term
(41), (19), (17), (15), (13), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(190), (75), (71), (16), (11), (10), (5), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(172), (19), (4), (2)
Clonality
Polyclonal
Conjugate This AIFM1 antibody is un-conjugated
(4), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(171), (82), (51), (42), (30), (21), (13), (11), (9), (6), (4), (4), (2), (2)
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Product Details anti-AIFM1 Antibody

Target Details AIFM1 Application Details Handling Images
Sequence EQHEDLNEVA KLFNIHED
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Apoptosis-inducing factor 1, mitochondrial(AIFM1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: apoptosis-inducing factor, mitochondrion-associated, 1
Protein Name: Apoptosis-inducing factor 1, mitochondrial
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human AIF(596-613aa EQHEDLNEVAKLFNIHED), identical to the related rat and mouse sequences.
Isotype IgG

Target Details AIFM1

Product Details anti-AIFM1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name AIFM1 (AIFM1 Antibody Abstract)
Background Apoptosis-inducing factor 1, mitochondrial, also known as AIF or PDCD8 is a protein that in humans is encoded by the AIFM1 gene. AIFM1 gene is mapped to Xq26.1 based on an alignment of the AIFM1 sequence with the genomic sequence. This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. A related pseudogene has been identified on chromosome 10.
UniProt O95831
Pathways Apoptosis

Application Details

Product Details anti-AIFM1 Antibody Target Details AIFM1 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1) (AA 596-613) (ABIN3044471) Anti-AIF antibody, IHC(P) IHC(P): Rat Cardiac Muscle Tissue
Western Blotting (WB) image for anti-AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1) (AA 596-613) (ABIN3044471) Anti-AIF antibody, Western blotting Lane 1: Rat Heart Tissue Lysate Lane 2: Rat Brain...
Immunohistochemistry (IHC) image for anti-AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1) (AA 596-613) (ABIN3044471) Anti-AIF antibody, IHC(P) IHC(P): Human Lung Cancer Tissue