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anti-Rat (Rattus) AIFM1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended AIFM1 Antibody (supplied by: Log in to see )

Antigen
Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) Antibodies
  • aif
  • Aif
  • AIF
  • AIFM1
  • AIFsh2
  • CG7263
  • CMTX4
  • COWCK
  • COXPD6
  • DDBDRAFT_0187853
  • DDBDRAFT_0191137
  • DDB_0187853
  • DDB_0191137
  • DmAIF
  • Dmel\\CG7263
  • GB16024
  • Hq
  • mAIF
  • PCD8
  • PDCD8
  • pdcd8
  • Pdcd8
  • zgc:91994
Epitope
Intermediate Domain
38
17
17
15
13
5
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
174
67
63
15
9
8
5
5
4
3
2
1
1
1
1
1
1
Host
Rabbit
160
17
2
2
Clonality
Polyclonal
Conjugate
This AIFM1 antibody is un-conjugated
4
4
4
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
155
74
46
35
27
18
11
10
8
5
4
3
2
Supplier
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Catalog No. ABIN499237
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089488085 ABIN724010 IF (p) IHC (p) WB Rabbit IgG AA 130-180 Log in to see Polyclonal 1
0.00089488085 ABIN3043779 ICC IHC (p) WB Rabbit IgG AA 582-613, C-Term Log in to see Polyclonal
0.00089488085 ABIN1105269 EIA IF IHC (p) WB Mouse IgG2b Log in to see 4-00E-007 1
0.00089488085 ABIN3044471 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 596-613, C-Term Log in to see Polyclonal
0.00089488085 ABIN261023 IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal
0.00089488085 ABIN724012 IHC (p) WB Biotin Rabbit IgG AA 130-180 Log in to see Polyclonal
0.00089488085 ABIN724019 IHC (p) WB HRP Rabbit IgG AA 130-180 Log in to see Polyclonal
0.00089488085 ABIN121584 IHC (p) WB Rabbit AA 517-531 Log in to see Polyclonal
0.00089488085 ABIN2477306 IHC (p) WB Rabbit IgG AA 517-531 Log in to see Polyclonal 1
0.00089488085 ABIN1731624 IHC (p) WB Rabbit IgG AA 151-180 Log in to see Polyclonal
0.00089488085 ABIN1731626 ICC IHC (p) ELISA WB Rabbit IgG AA 593-606 Log in to see Polyclonal
0.00089488085 ABIN320447 IHC (p) WB Rabbit IgG AA 517-531 Log in to see Polyclonal
0.00089488085 ABIN408863 IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-AIFM1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-AIFM1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 23 Antibodies 26 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 3 Antibodies
Flow Cytometry (FACS) 2 Antibodies 2 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 9 Antibodies 9 Antibodies
Immunofluorescence (IF) 7 Antibodies 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 21 Antibodies 23 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 54 Antibodies

General

Antigen Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) Antibodies
Epitope Intermediate Domain
(38), (17), (17), (15), (13), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(174), (67), (63), (15), (9), (8), (5), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(160), (17), (2), (2)
Clonality
Polyclonal
Conjugate This AIFM1 antibody is un-conjugated
(4), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(155), (74), (46), (35), (27), (18), (11), (10), (8), (5), (4), (3), (2)
Pubmed 3 references available
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Product Details anti-AIFM1 Antibody

Target Details AIFM1 Application Details Handling References for anti-AIFM1 Antibody (ABIN499237) Images
Specificity AIF antibody was raised against a peptide corresponding to amino acids 517 to 531 of human AIF.
Purification Ion exchange chromatography
Immunogen Human AIF (Intermediate Domain) Peptide
Isotype IgG

Target Details AIFM1

Product Details anti-AIFM1 Antibody Application Details Handling References for anti-AIFM1 Antibody (ABIN499237) Images back to top
Antigen
Alternative Name AIFM1 / AIF (AIFM1 Antibody Abstract)
Background Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli (2,3). AIF is highly conserved between human and mouse and widely expressed (2).Synonyms: Apoptosis-inducing factor 1 mitochondrial, PDCD8, Programmed cell death protein 8
Gene ID 9131
UniProt O95831
Pathways Apoptosis

Application Details

Product Details anti-AIFM1 Antibody Target Details AIFM1 Handling References for anti-AIFM1 Antibody (ABIN499237) Images back to top
Application Notes ELISA. Western Blot: AIF antibody can be used for detection of AIF at 0.25 to 1 μg/mL. A 67 kDaband should be detected. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details References for anti-AIFM1 Antibody (ABIN499237) Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-AIFM1 Antibody (ABIN499237)

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details Handling Images back to top
Background publications

Daugas, Susin, Zamzami, Ferri, Irinopoulou, Larochette, Prévost, Leber, Andrews, Penninger, Kroemer: "Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 14, Issue 5, pp. 729-39, 2000

Zamzami, Kroemer: "Condensed matter in cell death." in: Nature, Vol. 401, Issue 6749, pp. 127-8, 1999

Susin, Lorenzo, Zamzami, Marzo, Snow, Brothers, Mangion, Jacotot, Costantini, Loeffler, Larochette, Goodlett, Aebersold, Siderovski, Penninger, Kroemer: "Molecular characterization of mitochondrial apoptosis-inducing factor." in: Nature, Vol. 397, Issue 6718, pp. 441-6, 1999

Images

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details Handling References for anti-AIFM1 Antibody (ABIN499237) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1) (Intermediate Domain) (ABIN499237) Immunohistochemistry of AIF in human retina with AIF antibody at 10 μg/ml.
Western Blotting (WB) image for anti-AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1) (Intermediate Domain) (ABIN499237) Western blot analysis of AIF in K562 cell lysate (A), rat heart (B), and mouse heart ...